FastQCFastQC Report
Wed 25 May 2016
SRR1780892_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780892_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences314013
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTCTACCAATCTCGTATGCCGT201706.423300946139173No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCTACCAATCTCGTATGCCG33301.060465649511326No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCTACCAATCTCGTATGCC18540.5904214156738734No Hit
ATACACATCTCCGAGCCCACGAGACTTCTACCAATCTCGTATGCCGTCTT6290.20031017824102826Illumina PCR Primer Index 4 (95% over 22bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5070.1614582835742469No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTCTACCAATCTCGTATGCC5000.1592290765032021No Hit
CTTATACACATCTCCGCGCCCACGAGACTTCTACCAATCTCGTATGCCGT4760.1515860808310484No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCTACCAATATCGTATGCCGT3770.12005872368341439No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG24700.059.2361670
GACGTCC308.369358E-658.32935342
CCGACTT553.6379788E-1157.2688247
AAAACGG252.3872787E-456.00509667
AACGGGG252.3872787E-456.00509669
CGTATGA950.055.25938841
ACGTATG653.6379788E-1253.84247640
GACAACG200.006944854752.5047863
TGACGTC800.052.49641845
ATATCGT1600.052.49641837
GCCGACT608.367351E-1152.49641846
TGCCGAC800.052.49641845
CAGCTTG1250.050.40458754
ATCGTAT1600.050.3090739
GACTTAT352.0828564E-550.00454726
AAACTCG707.2759576E-1249.99658636
ACGTCCG352.08482E-549.99658643
CGTCATC1400.049.99658648
GCCGTCA1400.049.99658646
TATGCCG34000.049.8201343