FastQCFastQC Report
Wed 25 May 2016
SRR1780891_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780891_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences451296
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG318617.0598897397716796No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCATG150.00222348270.0043213
TGCGCGG200.00695066452.497425
GACGACA750.051.33081470
GTGCGCG305.8770087E-446.6643754
TCGCCGT659.997166E-943.07958252
GTCGCCG801.4224497E-939.37306651
TGGTCGC801.4224497E-939.37306649
GCGAGAT556.6822467E-638.18840838
GCCGTAT654.859012E-737.69463754
CGGTGGT1051.4551915E-1136.6648746
TCGGTGG1154.0017767E-1133.47661645
GTGGTCG1055.493348E-1033.331748
GATCCTA851.0619988E-732.943211
GATCGGC552.991456E-431.81662230
CTCGGTG1001.2560122E-831.50194744
TCTATCG450.004306749431.10958529
GGCCCTA802.4630353E-630.626891
CGCCGTA802.4630353E-630.6268953
CTGCGAG703.4820892E-530.00517740
CTACGGG500.007199731728.00483338
Produced by FastQC (version 0.11.5)