FastQCFastQC Report
Wed 25 May 2016
SRR1780891_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780891_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences451296
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCGT195324.327979862440615No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCG32910.7292331418847054No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCC13750.3046780826774445No Hit
ATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCGTCTT5470.12120648089059066RNA PCR Primer, Index 15 (95% over 22bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5080.11256470254555768No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCC4890.10835460540310574No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCGT150.00222444569.9966727
TCGTTAG150.00222444569.9966717
AACTCGT301.0232179E-769.9966737
AGGGGGG24850.060.84892370
TGACGTC700.059.9971545
GCCGACT553.6379788E-1157.27000446
TGCCGAC800.056.87229545
AAGCCGT509.804353E-1055.99734544
CGTATGA653.6379788E-1253.84359441
AAACTCG407.476592E-752.49750536
CGTTAGC200.006950620652.49750518
TACCGTG200.006950620652.4975057
TATGCCG34300.052.34445643
ATCTCGT33800.052.29041737
TCGTATG35250.052.2244740
CGTATGC35000.052.1975241
CTCGTAT33850.052.00639739
TCTCGTA34000.051.9828338
AATCTCG34050.051.9064936
CGTCTTC33550.051.7469448