FastQCFastQC Report
Wed 25 May 2016
SRR1780890_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780890_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58988
Sequences flagged as poor quality0
Sequence length76
%GC85

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3575460.61232793110462No Hit
CTTATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTG3100.5255306163965553No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1060.17969756560656405No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG890.15087814470739813No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG830.14070658439004544No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG800.1356208042313691No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG790.13392554417847696No Hit
GGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG750.12714450396690852No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG630.10680138333220317No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCCC150.002214420170.036
CCTATAG150.002214420170.031
GGTCCCT150.002214420170.051
TAGCCCC150.002214420170.035
ATGGTGT150.002214420170.010
GTCTGGC150.002214420170.010
GCCAGTG150.002214420170.045
CCCAAGC150.002214420170.040
ATAGCCC150.002214420170.034
CACCTAT150.002214420170.029
GCGCTGC207.866485E-570.015
CTGGAAT150.002214420170.056
GAATTGC150.002214420170.059
AGTTGGC150.002214420170.023
CGAGTAG150.002214420170.035
TGGAATT150.002214420170.057
CCCCCAA150.002214420170.038
TGGCGCT301.00752004E-770.013
ACCTATA150.002214420170.030
TGGTGTT150.002214420170.011
Produced by FastQC (version 0.11.5)