FastQCFastQC Report
Wed 25 May 2016
SRR1780890_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780890_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58988
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCCGT2250238.14674171017834No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCCG38976.606428426120567No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCC16392.7785312266901743No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6211.0527564928460025No Hit
ATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCCGTCTT6201.0510612327931106TruSeq Adapter, Index 8 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCC5410.9171356886146336No Hit
CTTATACACATCTCCGCGCCCACGAGACCATTTCGAATCTCGTATGCCGT4540.7696480640130196No Hit
CTTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGACGT4010.6797992812097375No Hit
CTTATACACATCTCCGAGCCCACGAGACCATTTCGAATATCGTATGCCGT2800.4746728148097918No Hit
CTTATACACATCTCCGAGCCCACGAGACCATATCGAATCTCGTATGCCGT2660.45093917406930223No Hit
CTTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTAAGCCGT2610.44246287380484167No Hit
CTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCCGTC2140.36278565131891233No Hit
CTTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCAGT2130.3610903912660202No Hit
CTTATACACATCTCCGAGCCCACGAGACAATTTCGAATCTCGTATGCCGT2050.34752831084288327No Hit
CTTATACACATCTCCGAGCCCACGAGACCAATTCGAATCTCGTATGCCGT1660.28141316878009087No Hit
CTTAAACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCCGT1630.27632738862141454No Hit
CTTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCCGA1560.2644605682511697No Hit
CTTTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCCG1490.2525937478809249No Hit
CTTATACACATCTCCGAGACCACGAGACCATTTCGAATCTCGTATGCCGT1480.2508984878280328No Hit
CTTATACACATCTCCGAGCCCACGAGACCATTTCGCATCTCGTATGCCGT1470.2492032277751407No Hit
CTTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGAATGCCGT1290.21868854682308264No Hit
CTTATACACATCTCCGAGCCCACGAGCCCATTTCGAATCTCGTATGCCGT1230.20851698650572997No Hit
TATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCCGTCT1180.2000406862412694No Hit
CTTATACACATCTCCGAGCCAACGAGACCATTTCGAATCTCGTATGCCGT1150.19495490608259308No Hit
CCTTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCCG1140.19325964602970094No Hit
CTTTACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCCGTC1130.19156438597680883No Hit
CTTATACACATCTCCGAGCCCACGAGACCATTTCGACTCTCGTATGCCGT1050.1780023055536719No Hit
TCTTATACACATCTCCGCGCCCACGAGACCATTTCGAATCTCGTATGCCG1040.17630704550077983No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCC1030.17461178544788772No Hit
CTTATACACATCTCCGAGCCCACGAGACCATTTCGAAACTCGTATGCCGT910.15426866481318235No Hit
CTTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTAGTATGCCGT890.15087814470739813No Hit
CTTATACACATCTCCGAGCACACGAGACCATTTCGAATCTCGTATGCCGT870.14748762460161388No Hit
TTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCCGTC860.14579236454872177No Hit
ATTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCCGT850.14409710449582966No Hit
CTTATAAACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCCGT730.1237539838611243No Hit
CTAATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCCGT730.1237539838611243No Hit
TCTTATACACATCTCCGAGCCCACGAGCCCATTTCGAATCTCGTATGCCG730.1237539838611243No Hit
CTTATACACATCTCCGAGCCCACGAGACCATTACGAATCTCGTATGCCGT710.12036346375534007No Hit
CTTATACACATCTCCGAGCCCACGAGACCATTTAGAATCTCGTATGCCGT710.12036346375534007No Hit
CTTATACACATCTCCGAGCCCACGAGACCATTTCGATCTCGTATGCCGTC690.11697294364955585No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCAG650.1101919034379874No Hit
TCCTTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCC650.1101919034379874No Hit
CATATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCCGT640.10849664338509526No Hit
GCTTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCCG640.10849664338509526No Hit
CTTATACACATCACCGAGCCCACGAGACCATTTCGAATCTCGTATGCCGT630.10680138333220317No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTCC252.8076774E-670.000019
ACACAAC252.8076774E-670.000016
CGTCATA301.00752004E-770.048
CCAACTC150.002214420170.037
CTATAGG150.002214420170.028
AATCACG301.00752004E-770.036
CAACTGC150.002214420170.051
CACGTAT353.6325218E-970.039
CAACTCT150.002214420170.038
ATAGGTG150.002214420170.030
CATCTGA207.866485E-570.051
ACGTATG401.3096724E-1070.040
AATTAGA150.002214420170.030
AACCCTA150.002214420170.028
TCAACTG150.002214420170.050
CACTGGC150.002214420170.014
TGATAGA150.002214420170.055
AGAATGA207.866485E-570.055
AGGGACC207.866485E-570.08
AGGTGCC150.002214420170.032
Produced by FastQC (version 0.11.5)