##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780890_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 58988 Sequences flagged as poor quality 0 Sequence length 76 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.511103953346442 32.0 32.0 32.0 27.0 32.0 2 30.690157320132908 32.0 32.0 32.0 27.0 32.0 3 30.71243303722791 32.0 32.0 32.0 27.0 32.0 4 30.182223503085375 32.0 32.0 32.0 27.0 32.0 5 30.536041228724486 32.0 32.0 32.0 27.0 32.0 6 32.65903234556181 37.0 32.0 37.0 13.0 37.0 7 34.226469790465856 37.0 37.0 37.0 27.0 37.0 8 31.51835966637282 37.0 32.0 37.0 13.0 37.0 9 33.79331389435139 37.0 37.0 37.0 27.0 37.0 10-11 33.56431816640673 37.0 34.5 37.0 20.0 37.0 12-13 34.440335322438465 37.0 37.0 37.0 27.0 37.0 14-15 33.997838543432565 37.0 37.0 37.0 27.0 37.0 16-17 31.486488777378447 37.0 27.0 37.0 17.5 37.0 18-19 32.963958771275514 37.0 32.0 37.0 22.0 37.0 20-21 33.76529972197735 37.0 37.0 37.0 24.5 37.0 22-23 32.54477181799688 37.0 32.0 37.0 20.0 37.0 24-25 33.49143893673289 37.0 32.0 37.0 24.5 37.0 26-27 33.28602427612396 37.0 34.5 37.0 20.0 37.0 28-29 33.2689614836916 37.0 34.5 37.0 22.0 37.0 30-31 33.47357089577541 37.0 34.5 37.0 24.5 37.0 32-33 33.370507560859835 37.0 32.0 37.0 22.0 37.0 34-35 33.77888723130128 37.0 34.5 37.0 24.5 37.0 36-37 31.625271241608463 37.0 29.5 37.0 13.0 37.0 38-39 31.499491421984132 37.0 29.5 37.0 13.0 37.0 40-41 32.521487421170406 37.0 32.0 37.0 13.0 37.0 42-43 32.74037939919984 37.0 32.0 37.0 17.5 37.0 44-45 32.90317522207907 37.0 32.0 37.0 17.5 37.0 46-47 31.880509595171898 37.0 29.5 37.0 13.0 37.0 48-49 31.08501729165254 37.0 27.0 37.0 13.0 37.0 50-51 31.8623109785041 37.0 29.5 37.0 13.0 37.0 52-53 30.671467078049773 37.0 27.0 37.0 13.0 37.0 54-55 30.477232657489658 37.0 27.0 37.0 13.0 37.0 56-57 30.537880585881872 37.0 27.0 37.0 13.0 37.0 58-59 30.48743812300807 37.0 24.5 37.0 13.0 37.0 60-61 31.392198413236592 37.0 29.5 37.0 13.0 37.0 62-63 33.12309283244049 37.0 32.0 37.0 22.0 37.0 64-65 33.461704075405166 37.0 32.0 37.0 22.0 37.0 66-67 33.248372550349224 37.0 32.0 37.0 22.0 37.0 68-69 32.23681935308876 37.0 32.0 37.0 13.0 37.0 70-71 27.724799959313756 32.0 20.0 37.0 10.5 37.0 72-73 29.58284735878484 37.0 20.0 37.0 13.0 37.0 74-75 31.780345154946765 37.0 32.0 37.0 13.0 37.0 76 32.743151149386314 37.0 32.0 37.0 13.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 11101 1 0.387311999566176 11101 2 -0.4047619459788443 11101 3 -0.05324407809359144 11101 4 -0.025398804207686254 11101 5 -0.062063746066129966 11101 6 1.4587285621812853 11101 7 -0.753760487160136 11101 8 -1.930800953296064 11101 9 -0.960640694221901 11101 10-11 -1.7971363607437318 11101 12-13 -1.1729743298042195 11101 14-15 -0.36131451672913784 11101 16-17 -0.3272571992804494 11101 18-19 -0.654128773571081 11101 20-21 0.03062975752860808 11101 22-23 0.5905485567089812 11101 24-25 -0.8750987130836521 11101 26-27 0.2502929380190295 11101 28-29 -1.1271903536715797 11101 30-31 -1.9741788446709094 11101 32-33 0.32020868023773374 11101 34-35 -1.1563726970385844 11101 36-37 -2.4853116050411366 11101 38-39 -2.673732523979922 11101 40-41 -1.1255260470728672 11101 42-43 0.26263183220920894 11101 44-45 -1.293271219722861 11101 46-47 -0.5875445372933079 11101 48-49 -0.015316803201123719 11101 50-51 -1.0263954504965795 11101 52-53 -2.4747316940723394 11101 54-55 -1.8301496926509593 11101 56-57 -3.49990938832153 11101 58-59 -2.1194474330425805 11101 60-61 -1.4518507321926535 11101 62-63 -0.2811519816849355 11101 64-65 -1.5976889440311766 11101 66-67 -0.26793688135976623 11101 68-69 -0.9408381484490249 11101 70-71 -0.8263592100283041 11101 72-73 -3.790583639366691 11101 74-75 -0.11115624102102828 11101 76 -1.5092024784488771 11102 1 -0.5571324448782704 11102 2 -0.37698416820106573 11102 3 0.3634225885730764 11102 4 -1.1087321375410184 11102 5 -1.2842859682883514 11102 6 -0.9579381044853825 11102 7 0.551795068395414 11102 8 -0.40302317551828537 11102 9 -2.4328629164441224 11102 10-11 -1.0332474718548426 11102 12-13 0.11869233686245195 11102 14-15 0.9997965943819693 11102 16-17 -0.20225719928044938 11102 18-19 -1.0152398846821882 11102 20-21 -0.1360369091380491 11102 22-23 0.048881890042309806 11102 24-25 -0.5973209353058735 11102 26-27 0.6669596046856938 11102 28-29 -0.016079242560465445 11102 30-31 0.6508211553290906 11102 32-33 -0.6936802086511591 11102 34-35 -1.5174838081496986 11102 36-37 -1.0964227161522473 11102 38-39 -1.5348436350910362 11102 40-41 1.4161406195937971 11102 42-43 0.02652072109809467 11102 44-45 -1.3071601086117468 11102 46-47 -1.3236556484044222 11102 48-49 0.17912764124331915 11102 50-51 0.07082677172564189 11102 52-53 -0.2663983607390037 11102 54-55 -0.2607052482065164 11102 56-57 1.291757278345134 11102 58-59 -0.7444474330425805 11102 60-61 -0.9101840655259856 11102 62-63 -1.628374203907157 11102 64-65 -0.7921333884756265 11102 66-67 -0.8929368813597662 11102 68-69 -1.0102825928934678 11102 70-71 0.9375296788605851 11102 72-73 -1.1516947504778017 11102 74-75 -2.180600685465471 11102 76 -1.0369802562266557 11103 1 -0.25908439683022166 11103 2 0.18007288885599237 11103 3 0.3619210870715719 11103 4 -0.00512853393741608 11103 5 -1.4509526349550192 11103 6 -3.3595897561370336 11103 7 -0.635141868541524 11103 8 -1.4585787310738425 11103 9 -3.688118171699376 11103 10-11 -1.0475117361191053 11103 12-13 -0.2747010565309438 11103 14-15 -1.2017799821946014 11103 16-17 -2.601656598679849 11103 18-19 0.9569823375400333 11103 20-21 -0.8413672394683829 11103 22-23 -1.738655647495225 11103 24-25 -1.0413900043749464 11103 26-27 -1.3431755304494395 11103 28-29 -1.7285417050229306 11103 30-31 -1.9220016674937312 11103 32-33 -0.9969835119544612 11103 34-35 -1.3354267510926334 11103 36-37 -0.05588217561170694 11103 38-39 -1.7495583498057492 11103 40-41 -1.6533038248506458 11103 42-43 -1.3890198194424457 11103 44-45 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24-25 0.3691823326679824 11104 26-27 -0.5021580423731322 11104 28-29 -0.6860138830833407 11104 30-31 -0.2936232891153523 11104 32-33 -1.460020077932203 11104 34-35 -0.5607844617444684 11104 36-37 1.0947537544359918 11104 38-39 -1.641052785417834 11104 40-41 -2.462127354262414 11104 42-43 -1.3631851612548473 11104 44-45 0.021271263937272522 11104 46-47 -3.362054341214879 11104 48-49 -1.0627024241161571 11104 50-51 -1.6178987184704354 11104 52-53 -2.2369865960331197 11104 54-55 -0.8023719148731807 11104 56-57 -1.650236185707147 11104 58-59 -2.603107563761533 11104 60-61 -1.1716219740227203 11104 62-63 -0.4960212627306859 11104 64-65 -1.971054957103071 11104 66-67 -0.796531652601594 11104 68-69 -1.5838120046581707 11104 70-71 -0.040411497610001845 11104 72-73 -1.753818933484336 11104 74-75 -3.1626268292563218 11104 76 -0.34580378563842373 11105 1 0.9706453328995082 11105 2 -0.3408730570899543 11105 3 -0.49213296698248143 11105 4 -0.950398804207687 11105 5 -0.62595263495502 11105 6 0.6309507844035096 11105 7 0.26012840172874974 11105 8 -0.4335787310738439 11105 9 -1.7273073608885667 11105 10-11 -1.2485252496326211 11105 12-13 -0.5493632186931023 11105 14-15 -0.03353673895136211 11105 16-17 0.8699650229417735 11105 18-19 1.2944823375400318 11105 20-21 -1.083259131360272 11105 22-23 0.4224930011534269 11105 24-25 -2.315376490861432 11105 26-27 0.24195960468569666 11105 28-29 -1.0771903536715826 11105 30-31 0.31887671088464487 11105 32-33 -0.4159024308733805 11105 34-35 0.09362730296141564 11105 36-37 0.45357728384774987 11105 38-39 -1.4542880795354804 11105 40-41 0.5591961751493528 11105 42-43 -0.4859792789019082 11105 44-45 -1.8779934419450797 11105 46-47 -1.7958778706266436 11105 48-49 0.5277387523544306 11105 50-51 0.754160105058979 11105 52-53 -0.3038983607390051 11105 54-55 1.6851280851268164 11105 56-57 -2.1443538327659724 11105 58-59 -1.6722252108203577 11105 60-61 -0.11573962108154134 11105 62-63 0.48412579609284023 11105 64-65 -1.5754667218089544 11105 66-67 0.40567422975134804 11105 68-69 0.2897174071065294 11105 70-71 0.35002967886058656 11105 72-73 1.7469163606333105 11105 74-75 -0.03615624102102544 11105 76 1.5130197437733415 11106 1 0.14711592113480165 11106 2 -0.618814233560542 11106 3 -1.2509564963942452 11106 4 -0.8636340983253348 11106 5 0.10787089445674525 11106 6 -1.8484609803023773 11106 7 -0.5413421865065473 11106 8 -0.04681402519149103 11106 9 -0.17142500794739135 11106 10-11 -0.5904370143385052 11106 12-13 -0.9515691010460472 11106 14-15 -0.8408896801278303 11106 16-17 -0.7153290947052859 11106 18-19 0.4863941022459173 11106 20-21 -1.8362003078308646 11106 22-23 0.351169471741656 11106 24-25 0.016241156197395412 11106 26-27 -0.016863924726067125 11106 28-29 -0.30366094190686965 11106 30-31 0.2063767108846477 11106 32-33 -1.1511965485204385 11106 34-35 0.2774508323731837 11106 36-37 0.021224342671281704 11106 38-39 -0.2881116089472471 11106 40-41 0.9790491163258253 11106 42-43 2.4162266034510367 11106 44-45 0.021271263937265417 11106 46-47 -0.1708778706266436 11106 48-49 -1.1656436005867477 11106 50-51 -0.8237810714116094 11106 52-53 -0.7075748313272392 11106 54-55 0.9035104380679932 11106 56-57 0.849763814292853 11106 58-59 -0.6325193284674171 11106 60-61 -0.45103373872860075 11106 62-63 -1.554844792142454 11106 64-65 -1.7798784865148356 11106 66-67 -0.664178711425123 11106 68-69 -1.1573414164228808 11106 70-71 1.2537061494488206 11106 72-73 1.334416360633309 11106 74-75 0.08737317074367468 11106 76 -1.3458037856384202 11107 1 -0.5781351549053682 11107 2 0.16095621120272696 11107 3 -0.009206137714187435 11107 4 -1.17783782859793 11107 5 -0.5972940983696517 11107 6 -2.3556345814501505 11107 7 0.26927474319216316 11107 8 -2.190286048147012 11107 9 0.12269263911143469 11107 10-11 -0.6220008593887201 11107 12-13 -0.7783266333272536 11107 14-15 -0.10487820236598822 11107 16-17 -0.6629618063265248 11107 18-19 -0.6405786380697194 11107 20-21 0.57161891742021 11107 22-23 -0.9436655354319399 11107 24-25 -1.1254374664711833 11107 26-27 -0.012918444094793813 11107 28-29 0.3423218414503708 11107 30-31 -1.0192330452129141 11107 32-33 -0.7695609674587445 11107 34-35 -0.7447873311849307 11107 36-37 0.7694309423843393 11107 38-39 -0.47928807953547903 11107 40-41 0.24913519953960162 11107 42-43 -0.43384513256044244 11107 44-45 -1.524639783408496 11107 46-47 -1.0916095779437178 11107 48-49 -1.624395393987033 11107 50-51 0.2730625440833663 11107 52-53 -1.376154458299979 11107 54-55 -0.8572499636536683 11107 56-57 -1.5845977352049978 11107 58-59 -2.7021032596008467 11107 60-61 -1.0267152308376417 11107 62-63 0.40821116194649676 11107 64-65 -1.2748569657113933 11107 66-67 -1.9769477214681643 11107 68-69 1.5567905778382354 11107 70-71 -0.22405568699307565 11107 72-73 -0.7143641271715673 11107 74-75 -0.8642050215088339 11107 76 1.017897792553832 11108 1 0.49564533289950674 11108 2 -1.9158730570899536 11108 3 -1.9421329669824807 11108 4 -1.6753988042076848 11108 5 -1.8259526349550192 11108 6 -0.09404921559649182 11108 7 -0.7898715982712474 11108 8 1.016421268926159 11108 9 -1.402307360888564 11108 10-11 -0.4610252496326268 11108 12-13 -0.48686321869310234 11108 14-15 -0.02103673895135927 11108 16-17 0.582465022941772 11108 18-19 0.7069823375400333 11108 20-21 0.3417408686397252 11108 22-23 1.097493001153424 11108 24-25 0.6596235091385694 11108 26-27 0.6044596046856938 11108 28-29 0.13530964632842313 11108 30-31 0.7063767108846477 11108 32-33 0.20909756912661948 11108 34-35 -0.5063726970385858 11108 36-37 1.0785772838477499 11108 38-39 1.0707119204645181 11108 40-41 0.34669617514935425 11108 42-43 0.8390207210980947 11108 44-45 -1.0154934419450825 11108 46-47 1.7166221293733592 11108 48-49 -0.409761247645573 11108 50-51 -1.5958398949410224 11108 52-53 -1.0538983607390016 11108 54-55 1.7601280851268193 11108 56-57 2.1431461672340326 11108 58-59 -0.2972252108203577 11108 60-61 -0.8657396210815413 11108 62-63 0.1591257960928445 11108 64-65 1.049533278191042 11108 66-67 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1.7714298216810498 11109 48-49 1.7902387523544299 11109 50-51 0.3753139512128243 11109 52-53 0.2977042033635584 11109 54-55 1.0245511620498995 11109 56-57 -0.053328191740330766 11109 58-59 0.8591850455898964 11109 60-61 -1.5298421851841049 11109 62-63 -1.1540152295481825 11109 64-65 0.787994816652585 11109 66-67 -1.0895180779409586 11109 68-69 0.8038199712091014 11109 70-71 1.0945809609118697 11109 72-73 0.24467277088971784 11109 74-75 1.590766835902052 11109 76 -1.6074930767394768 11110 1 0.049592701320559485 11110 2 -0.05534674130048245 11110 3 -0.3763434932982719 11110 4 -0.3017145936813712 11110 5 0.3648368387291896 11110 6 -0.8190492155964932 11110 7 -0.18066107195546266 11110 8 -2.1427892573896337 11110 9 -0.1404652556254078 11110 10-11 -2.389972618053676 11110 12-13 0.03682099183320986 11110 14-15 0.19935799789074338 11110 16-17 0.8489123913628269 11110 18-19 -1.950912399302073 11110 20-21 -1.1543117629392228 11110 22-23 -1.2479017356886821 11110 24-25 -2.1989291224403757 11110 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38-39 1.7575540257276785 21612 40-41 1.7940645962019843 21612 42-43 2.546257563203355 21612 44-45 1.6489802422654378 21612 46-47 1.539648445162829 21612 48-49 1.5533966470912723 21612 50-51 2.23968642084845 21612 52-53 -0.02955625547584617 21612 54-55 0.6844701903899768 21612 56-57 1.5951198514445544 21612 58-59 1.9054063681270108 21612 60-61 2.442155115760567 21612 62-63 0.003204743461267867 21612 64-65 2.5126911729278874 21612 66-67 0.8892268613302932 21612 68-69 1.7134016176328473 21612 70-71 0.45726652096584885 21612 72-73 1.1765216237912028 21612 74-75 1.7506858642421292 21612 76 1.397230270089132 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 1.0 17 2.0 18 9.0 19 23.0 20 59.0 21 116.0 22 243.0 23 468.0 24 704.0 25 1060.0 26 1438.0 27 1989.0 28 2614.0 29 3385.0 30 4294.0 31 5694.0 32 7661.0 33 9975.0 34 11213.0 35 7192.0 36 848.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 11.466458658346333 7.891880892627009 14.527233263243572 66.11442718578309 2 6.562685428498771 8.09697380690006 68.3038060523862 17.03653471221497 3 6.511493863158608 10.220722858886553 72.6554553468502 10.612327931104632 4 4.555240985301847 60.78966551952125 25.247935985895197 9.407157509281706 5 4.607716823760765 23.2369295449922 66.84071336543026 5.314640265816776 6 8.820587587095462 69.6289012833336 14.99482936918304 6.555681760387882 7 7.852843943375435 21.342714249385438 11.475798931931847 59.32864287530728 8 6.748830270563505 69.57177731063945 7.4727063131484375 16.206686105648608 9 6.928762757264435 16.91926897907978 5.987861526463907 70.16410673719187 10-11 7.5100020343120635 42.26113785854751 35.137655116294845 15.091204990845597 12-13 8.030075697852826 9.515211623392586 42.38486382015614 40.06984885859845 14-15 8.430528243032482 8.025361090391266 13.860446192445922 69.68366447413034 16-17 40.442304595119225 34.15696811982403 6.060708806252278 19.34001847880447 18-19 42.36370109174747 15.733708550891706 6.548789584322234 35.353800773038586 20-21 10.627188037738087 8.470725856354527 6.398182604199337 74.50390350170805 22-23 8.702617481521665 40.52264867430664 5.9724011663389165 44.80233267783278 24-25 40.329389028276935 38.442564589408015 6.346206008001627 14.881840374313422 26-27 42.323373596100865 43.33206187751642 6.396270396270396 7.948294130112312 28-29 9.863441015164744 16.218392655822196 8.274915021488331 65.64325130752474 30-31 6.56489455482471 40.09798603105716 35.80558757713433 17.531531836983795 32-33 7.744861199406654 11.784700148336512 74.00296673023946 6.467471922017376 34-35 34.76978368481725 8.6093781786126 18.545297348613275 38.075540787956875 36-37 14.836915982911778 66.51691869532786 8.249135417373026 10.397029904387333 38-39 7.610280405519953 18.677313260773744 39.52124232868816 34.19116400501814 40-41 7.479867762990591 8.19021785199627 43.043994235822666 41.285920149190474 42-43 39.532104259376986 7.785547785547786 38.42424242424242 14.258105530832804 44-45 9.995337995337996 39.61941089213816 42.31828777283323 8.066963339690613 46-47 39.03369357914813 10.67683831320195 16.432294977749525 33.8571731299004 48-49 36.44978639723334 7.512544924391401 9.295958500033905 46.74171017834136 50-51 13.713423524226087 8.03580510629641 40.84443766317431 37.406333706303194 52-53 6.540535042213406 10.258366392025227 68.40606245549792 14.795036110263451 54-55 6.011545014537225 10.74906970238953 44.42711467878243 38.81227060429081 56-57 38.07820433489019 8.084900782382409 16.736032821069227 37.10086206165818 58-59 9.286023073076043 12.260432471836777 63.883261424224195 14.570283030862994 60-61 37.60510612327931 37.69241201600326 17.769715874415134 6.932765986302298 62-63 8.235573336949887 77.80141723740421 6.597952125856106 7.365057299789787 64-65 5.469048002509049 80.76593796885729 6.422656031464827 7.342357997168844 66-67 5.596243176346929 80.73271623775133 5.971756009900655 7.699284576001085 68-69 7.476160203432931 79.1176096630642 5.668997668997669 7.737232464505192 70-71 28.872952904078936 57.33733428271115 5.891228427084393 7.898484386125522 72-73 75.21445039669086 10.983589882688003 6.002915847290975 7.799043873330169 74-75 79.40291422613649 6.768497876632789 6.179380027633441 7.649207869597281 76 79.91997829919978 6.415299064152991 6.089787060897871 7.574935575749356 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 1.5 12 2.0 13 3.0 14 3.0 15 3.0 16 5.0 17 6.0 18 7.0 19 23.0 20 34.5 21 31.0 22 39.5 23 58.0 24 72.0 25 76.0 26 92.5 27 132.0 28 196.0 29 237.0 30 248.5 31 284.5 32 308.0 33 307.0 34 380.0 35 467.0 36 520.0 37 559.0 38 673.0 39 793.0 40 918.5 41 1259.0 42 1480.0 43 1868.5 44 3439.0 45 9182.0 46 13743.0 47 17318.5 48 11736.0 49 1974.5 50 1371.0 51 1015.5 52 639.0 53 600.0 54 582.0 55 596.0 56 606.0 57 537.0 58 472.0 59 447.0 60 371.5 61 291.5 62 262.0 63 225.0 64 174.0 65 139.0 66 114.0 67 110.0 68 98.5 69 89.0 70 72.5 71 54.0 72 46.5 73 44.5 74 41.5 75 33.0 76 23.0 77 14.5 78 22.0 79 28.0 80 16.0 81 7.0 82 8.0 83 6.0 84 6.5 85 6.0 86 4.5 87 4.0 88 3.0 89 4.5 90 4.5 91 3.0 92 4.0 93 3.0 94 4.0 95 6.0 96 6.0 97 12.0 98 33.5 99 321.5 100 594.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.027124160846273816 2 0.005085780158676341 3 0.0 4 0.0016952600528921135 5 0.0 6 0.0016952600528921135 7 0.005085780158676341 8 0.0 9 0.003390520105784227 10-11 0.0 12-13 0.0059334101851223975 14-15 0.0 16-17 0.0025428900793381705 18-19 0.0 20-21 0.0042381501322302844 22-23 0.0 24-25 0.0 26-27 8.476300264460568E-4 28-29 0.0042381501322302844 30-31 0.0 32-33 8.476300264460568E-4 34-35 0.0 36-37 0.0 38-39 0.003390520105784227 40-41 0.005085780158676341 42-43 8.476300264460568E-4 44-45 8.476300264460568E-4 46-47 8.476300264460568E-4 48-49 0.0 50-51 0.003390520105784227 52-53 0.003390520105784227 54-55 0.0025428900793381705 56-57 0.0025428900793381705 58-59 0.0025428900793381705 60-61 0.0 62-63 0.0 64-65 0.0025428900793381705 66-67 0.003390520105784227 68-69 8.476300264460568E-4 70-71 0.003390520105784227 72-73 0.0 74-75 0.0025428900793381705 76 0.006781040211568454 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 58988.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.759069641282974 #Duplication Level Percentage of deduplicated Percentage of total 1 76.0542276132715 18.06977690377704 2 8.697823760256867 4.133044008950973 3 5.465572600784873 3.895707601546077 4 3.8458794149125937 3.654980674035397 5 2.368890474491616 2.8141316878009084 6 1.1987156617909382 1.7088221333152507 7 0.5993578308954691 0.9968129111005628 8 0.46378879771673204 0.8815352275038992 9 0.1855155190866928 0.39669085237675455 >10 0.7563325008919015 3.120973757374381 >50 0.1569746699964324 2.5751000203431205 >100 0.1641098822689975 7.199769444632807 >500 0.021405636817695327 3.0209534142537464 >1k 0.014270424545130217 9.38495965281074 >5k 0.0 0.0 >10k+ 0.007135212272565109 38.14674171017834 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCCGT 22502 38.14674171017834 No Hit TCTTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCCG 3897 6.606428426120567 No Hit CTCTTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCC 1639 2.7785312266901743 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 621 1.0527564928460025 No Hit ATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCCGTCTT 620 1.0510612327931106 TruSeq Adapter, Index 8 (95% over 21bp) TCTTTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCC 541 0.9171356886146336 No Hit CTTATACACATCTCCGCGCCCACGAGACCATTTCGAATCTCGTATGCCGT 454 0.7696480640130196 No Hit CTTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGACGT 401 0.6797992812097375 No Hit CTTATACACATCTCCGAGCCCACGAGACCATTTCGAATATCGTATGCCGT 280 0.4746728148097918 No Hit CTTATACACATCTCCGAGCCCACGAGACCATATCGAATCTCGTATGCCGT 266 0.45093917406930223 No Hit CTTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTAAGCCGT 261 0.44246287380484167 No Hit CTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCCGTC 214 0.36278565131891233 No Hit CTTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCAGT 213 0.3610903912660202 No Hit CTTATACACATCTCCGAGCCCACGAGACAATTTCGAATCTCGTATGCCGT 205 0.34752831084288327 No Hit CTTATACACATCTCCGAGCCCACGAGACCAATTCGAATCTCGTATGCCGT 166 0.28141316878009087 No Hit CTTAAACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCCGT 163 0.27632738862141454 No Hit CTTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCCGA 156 0.2644605682511697 No Hit CTTTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCCG 149 0.2525937478809249 No Hit CTTATACACATCTCCGAGACCACGAGACCATTTCGAATCTCGTATGCCGT 148 0.2508984878280328 No Hit CTTATACACATCTCCGAGCCCACGAGACCATTTCGCATCTCGTATGCCGT 147 0.2492032277751407 No Hit CTTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGAATGCCGT 129 0.21868854682308264 No Hit CTTATACACATCTCCGAGCCCACGAGCCCATTTCGAATCTCGTATGCCGT 123 0.20851698650572997 No Hit TATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCCGTCT 118 0.2000406862412694 No Hit CTTATACACATCTCCGAGCCAACGAGACCATTTCGAATCTCGTATGCCGT 115 0.19495490608259308 No Hit CCTTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCCG 114 0.19325964602970094 No Hit CTTTACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCCGTC 113 0.19156438597680883 No Hit CTTATACACATCTCCGAGCCCACGAGACCATTTCGACTCTCGTATGCCGT 105 0.1780023055536719 No Hit TCTTATACACATCTCCGCGCCCACGAGACCATTTCGAATCTCGTATGCCG 104 0.17630704550077983 No Hit TCTTAATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCC 103 0.17461178544788772 No Hit CTTATACACATCTCCGAGCCCACGAGACCATTTCGAAACTCGTATGCCGT 91 0.15426866481318235 No Hit CTTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTAGTATGCCGT 89 0.15087814470739813 No Hit CTTATACACATCTCCGAGCACACGAGACCATTTCGAATCTCGTATGCCGT 87 0.14748762460161388 No Hit TTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCCGTC 86 0.14579236454872177 No Hit ATTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCCGT 85 0.14409710449582966 No Hit CTTATAAACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCCGT 73 0.1237539838611243 No Hit CTAATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCCGT 73 0.1237539838611243 No Hit TCTTATACACATCTCCGAGCCCACGAGCCCATTTCGAATCTCGTATGCCG 73 0.1237539838611243 No Hit CTTATACACATCTCCGAGCCCACGAGACCATTACGAATCTCGTATGCCGT 71 0.12036346375534007 No Hit CTTATACACATCTCCGAGCCCACGAGACCATTTAGAATCTCGTATGCCGT 71 0.12036346375534007 No Hit CTTATACACATCTCCGAGCCCACGAGACCATTTCGATCTCGTATGCCGTC 69 0.11697294364955585 No Hit TCTTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCAG 65 0.1101919034379874 No Hit TCCTTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCC 65 0.1101919034379874 No Hit CATATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCCGT 64 0.10849664338509526 No Hit GCTTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCCG 64 0.10849664338509526 No Hit CTTATACACATCACCGAGCCCACGAGACCATTTCGAATCTCGTATGCCGT 63 0.10680138333220317 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0016952600528921137 0.0 2 0.0 0.0 0.0 0.0016952600528921137 0.0 3 0.0 0.0 0.0 0.0016952600528921137 0.0 4 0.0 0.0 0.0 0.015257340476029023 0.0 5 0.0 0.0 0.0 0.02881942089916593 0.0 6 0.0 0.0 0.0 0.02881942089916593 0.0 7 0.0 0.0 0.0 0.02881942089916593 0.0 8 0.0 0.0 0.0 0.02881942089916593 0.0 9 0.0 0.0 0.0 0.02881942089916593 0.0 10 0.0 0.0 0.0 0.02881942089916593 0.0 11 0.0 0.0 0.0 0.02881942089916593 0.0 12 0.0 0.0 0.0 0.02881942089916593 0.0 13 0.0 0.0 0.0 0.02881942089916593 0.0 14 0.0 0.0 0.0 0.04068624126941073 0.0 15 0.0 0.0 0.0 0.04068624126941073 0.0 16 0.0 0.0 0.0 0.04238150132230284 0.0 17 0.0 0.0 0.0 0.04238150132230284 0.0 18 0.0 0.0 0.0 0.04238150132230284 0.0 19 0.0 0.0 0.0 0.04577202142808707 0.0 20 0.0 0.0 0.0 0.04746728148097918 0.0 21 0.0 0.0 0.0 0.0627246219570082 0.0 22 0.0 0.0 0.0 0.08306774259171357 0.0 23 0.0 0.0 0.0 0.10680138333220315 0.0 24 0.0 0.0 0.0 0.11866820370244796 0.0 25 0.0 0.0 0.0 0.13901132433715332 0.0 26 0.0 0.0 0.0 0.13901132433715332 0.0 27 0.0 0.0 0.0 0.15257340476029022 0.0 28 0.0 0.0 0.0 0.1881738658710246 0.0 29 0.0 0.0 0.0 0.20343120634705364 0.0 30 0.0 0.0 0.0 0.22886010714043534 0.0 31 0.0 0.0 0.0 0.2356411473520038 0.0 32 0.0 0.0 0.0 0.25937478809249337 0.0 33 0.0 0.0 0.0 0.3390520105784227 0.0 34 0.0 0.0 0.0 0.37973825184783344 0.0 35 0.0 0.0 0.0 0.5272258764494473 0.0 36 0.0 0.0 0.0 0.7560859835898827 0.0 37 0.0 0.0 0.0 0.969688750254289 0.0 38 0.0 0.0 0.0 1.3070455007798196 0.0 39 0.0 0.0 0.0 1.6732216722045161 0.0 40 0.0 0.0 0.0 1.9715874415135282 0.0 41 0.0 0.0 0.0 2.256391130399403 0.0 42 0.0 0.0 0.0 2.7683596663728216 0.0 43 0.0 0.0 0.0 3.1972604597545264 0.0 44 0.0 0.0 0.0 3.5702176713907914 0.0 45 0.0 0.0 0.0 4.068624126941073 0.0 46 0.0 0.0 0.0 4.455143419000475 0.0 47 0.0 0.0 0.0 4.86878687190615 0.0 48 0.0 0.0 0.0 5.234963043330847 0.0 49 0.0 0.0 0.0 5.645215976130738 0.0 50 0.0 0.0 0.0 6.058859429036414 0.0 51 0.0 0.0 0.0 6.42503560046111 0.0 52 0.0 0.0 0.0 6.675934088289144 0.0 53 0.0 0.0 0.0 6.965823557333695 0.0 54 0.0 0.0 0.0 7.235369905743541 0.0 55 0.0 0.0 0.0 7.60324133722113 0.0 56 0.0 0.0 0.0 7.8880450261070045 0.0 57 0.0 0.0 0.0 8.191496575574693 0.0 58 0.0 0.0 0.0 8.459347663931647 0.0 59 0.0 0.0 0.0 8.696684071336543 0.0 60 0.0 0.0 0.0 8.917067878212517 0.0 61 0.0 0.0 0.0 9.130670644876924 0.0 62 0.0 0.0 0.0 9.34427341154133 0.0 63 0.0 0.0 0.0 9.564657218417306 0.0 64 0.0 0.0 0.0 9.72062114328338 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AATCTCC 25 2.8076774E-6 70.00001 9 ACACAAC 25 2.8076774E-6 70.00001 6 CGTCATA 30 1.00752004E-7 70.0 48 CCAACTC 15 0.0022144201 70.0 37 CTATAGG 15 0.0022144201 70.0 28 AATCACG 30 1.00752004E-7 70.0 36 CAACTGC 15 0.0022144201 70.0 51 CACGTAT 35 3.6325218E-9 70.0 39 CAACTCT 15 0.0022144201 70.0 38 ATAGGTG 15 0.0022144201 70.0 30 CATCTGA 20 7.866485E-5 70.0 51 ACGTATG 40 1.3096724E-10 70.0 40 AATTAGA 15 0.0022144201 70.0 30 AACCCTA 15 0.0022144201 70.0 28 TCAACTG 15 0.0022144201 70.0 50 CACTGGC 15 0.0022144201 70.0 14 TGATAGA 15 0.0022144201 70.0 55 AGAATGA 20 7.866485E-5 70.0 55 AGGGACC 20 7.866485E-5 70.0 8 AGGTGCC 15 0.0022144201 70.0 32 >>END_MODULE