FastQCFastQC Report
Wed 25 May 2016
SRR1780889_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780889_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85120
Sequences flagged as poor quality0
Sequence length76
%GC69

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3075936.1360432330827No Hit
CTTATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTG2180.256109022556391No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTGGC150.002214413170.0270517
CGAACTG150.00221958569.98595
ATTCCTC150.00221958569.985910
TACTGCG150.00221958569.985932
GGGTTAC150.00221958569.985925
CGCGTCT150.00221958569.98597
AGATGTA150.00221958569.985936
CCGGCGG252.821811E-669.9858921
GCCGGCG352.9471994E-759.98791520
GGTCGGC252.3808033E-455.98871650
GGAGCGT200.00691945852.5202841
CATTCAC200.00691945852.5202813
TAACCCA200.00691945852.5202817
GAGCGTT200.00691945852.5202842
CGGCTGA200.00691945852.5202815
ATCTGTA200.00691945852.5202839
TCTCTCA200.006935572752.4894229
GGCCAGT200.006935572752.4894250
AATTGAG200.006935572752.4894267
AATTCCA200.006935572752.4894220
Produced by FastQC (version 0.11.5)