FastQCFastQC Report
Wed 25 May 2016
SRR1780889_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780889_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85120
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTATGCCGT1708920.076362781954888No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTATGCCG32363.801691729323308No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTATGCC17172.0171522556390977No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGCCCGAATCTCGTATGCCGT11421.3416353383458648No Hit
ATACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTATGCCGTCTT5690.6684680451127819RNA PCR Primer, Index 40 (95% over 21bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4600.5404135338345865No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTATGCC4250.49929511278195493No Hit
CTTATACACATCTCCGCGCCCACGAGACCTGACCGAATCTCGTATGCCGT3340.3923872180451128No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTAAGCCGT2860.3359962406015038No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTATGACGT2690.31602443609022557No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGACAGAATCTCGTATGCCGT2230.2619830827067669No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTGCCCGAATCTCGTATGCCG2180.256109022556391No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGACCGAATATCGTATGCCGT1720.20206766917293234No Hit
CTATACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTATGCCGTC1720.20206766917293234No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTATGCCGA1710.20089285714285712No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTATGCAGT1590.18679511278195488No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGACCGCATCTCGTATGCCGT1560.18327067669172933No Hit
CTTAAACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTATGCCGT1160.1362781954887218No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGAACGAATCTCGTATGCCGT1110.13040413533834588No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGAATGCCGT1110.13040413533834588No Hit
CTTATACACATCTCCGAGACCACGAGACCTGACCGAATCTCGTATGCCGT1090.1280545112781955No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGACCGAATCTAGTATGCCGT1060.12453007518796994No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTGCCCGAATCTCGTATGCC1060.12453007518796994No Hit
CTTATACACATCTCCGAGCCCACGAGCCCTGACCGAATCTCGTATGCCGT1020.11983082706766918No Hit
CTTTATACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTATGCCG1000.1174812030075188No Hit
TATACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTATGCCGTCT870.10220864661654135No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGTTT150.002213676270.0329370
GGGTAAC150.002218846569.99177613
AAGACGA150.002218846569.99177644
CGACCAA150.002218846569.99177612
GCAGATG150.002218846569.99177623
AGGGGGG20150.063.4293270
CTCTCGT453.783498E-1062.21491637
ACTCTCG401.0524673E-861.242836
CTATACA700.059.992951
CACCGAG352.9454895E-759.9929512
TGAACGA308.319674E-658.32647730
GACCACG601.8189894E-1258.32647718
AGACCGA601.8189894E-1258.32647730
AAGCCGT850.057.6402844
TCTTTAT800.056.8683171
CAGCATG1000.056.02633754
AGCCGAC252.3798157E-455.9934245
AATCACG252.3798157E-455.9934236
CATCTCG252.3798157E-455.9934236
GAGGTGT252.3798157E-455.9934216
Produced by FastQC (version 0.11.5)