FastQCFastQC Report
Wed 25 May 2016
SRR1780888_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780888_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences215559
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2601512.068621583881907No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGAT150.002222942469.9960643
GGCACTC150.002222942469.996061
CGCTGCG150.002222942469.9960616
AACCGGC200.006945963552.4970423
ACCGGCT200.006945963552.4970424
CTCGTCC200.006945963552.4970435
ACATTCC200.006945963552.4970418
TGCACGT200.006945963552.497045
TAGCGCC305.870349E-446.6640416
CGCTGGC451.6800295E-646.66403616
TCGCCGT551.304179E-744.55327652
GCGCTGG404.5838562E-543.7475315
TCGGTGG659.964424E-943.07449745
GTCGCCG602.587858E-740.83103651
ATAGCGC350.00125384839.99774615
TCCCGGA350.00125384839.99774633
TGCCGGC556.676506E-638.17966519
GCCGTAT654.838985E-737.6989354
GCGCTGC852.561137E-937.05673615
CATTGAA400.002415757634.99802826
Produced by FastQC (version 0.11.5)