FastQCFastQC Report
Wed 25 May 2016
SRR1780888_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780888_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences215559
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGCCGTGCATCTCGTATGCCGT158567.355758748184951No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGCCGTGCATCTCGTATGCCG27421.2720415292332956No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGCCGTGCATCTCGTATGCC13130.6091139780756081No Hit
ATACACATCTCCGAGCCCACGAGACCGCCGTGCATCTCGTATGCCGTCTT5210.2416971687565817RNA PCR Primer, Index 16 (95% over 23bp)
CTTATACACATCTCCGCGCCCACGAGACCGCCGTGCATCTCGTATGCCGT4290.19901743838113928No Hit
CTTATACACATCTCCGAGCCCACGAGCCCGCCGTGCATCTCGTATGCCGT3740.17350238217842912No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCGCCGTGCATCTCGTATGCC3630.16839937093788707No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3350.15540988778014372No Hit
CTTATACACATCTCCGAGCCCACGAGACCGCCGTGCATCTCGTATGACGT2170.10066849447251101No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGTC700.064.9946145
AGGGGGG18850.062.95368270
GCCGTGA453.8380676E-1062.2170730
CGTGAAT401.0624717E-861.24492632
CACGTAT401.0624717E-861.24492639
TGCCGTA401.0624717E-861.24492645
CCGACTT700.059.9950347
CTCGTAA900.058.32850339
CGAATGC601.8189894E-1258.328541
AAGCCGT800.056.8702944
CGTCTAC509.767973E-1055.9953648
GCCGTAT252.387643E-455.9953646
GTCTTCA1250.055.99535849
ATCGTAT452.6915586E-854.43993839
CAACTCG452.6915586E-854.43993836
GCCGACT800.052.4956546
AACTCGT407.460021E-752.4956537
CGACTTA200.00694669152.4956548
GTGGTAC200.00694669152.495653
GCCGTCA2000.052.4956546