FastQCFastQC Report
Wed 25 May 2016
SRR1780887_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780887_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88518
Sequences flagged as poor quality0
Sequence length76
%GC69

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3219936.37565240967939No Hit
CTTATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTG2330.26322329921597865No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATC1300.1468627849702885No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTGTA150.002218106870.02
GTTTCTT150.002218106870.030
CTGAGCG150.002218106870.09
CATAGGT150.002218106870.02
GGGCCCT150.002218106870.017
TGAGCGG207.888315E-570.010
CGTCAGA150.002218106870.017
GACTATC150.002218106870.038
TGCGTCA150.002218106870.015
GCGTCAG308.3166215E-658.33333216
AGACGCC252.3789558E-456.00000418
CTGGCGG452.665547E-854.44444718
CTTGTGC200.0069309652.51
GGATGTC200.0069309652.525
CAACAAG200.0069309652.570
TCGGCTT200.0069309652.526
GAGCGGC200.0069309652.511
TACTGAC200.0069309652.534
CTGCCGG200.0069309652.518
ACAGCCC200.0069309652.547
Produced by FastQC (version 0.11.5)