FastQCFastQC Report
Wed 25 May 2016
SRR1780887_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780887_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88518
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCCGT2036123.002101267538805No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCCG35153.9709437628504936No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCC15301.728462007727242No Hit
ATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCCGTCTT5570.6292505479111593RNA PCR Primer, Index 45 (95% over 22bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5210.5885808536116948No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCC4880.5513003005038524No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGACGT4310.4869066178630335No Hit
CTTATACACATCTCCGCGCCCACGAGACGCCAATTCATCTCGTATGCCGT3480.393140378228157No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCAGT2800.31631984455139067No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCAATTCATATCGTATGCCGT2600.293725569940577No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCCGA2350.26548272667706No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCAAATCATCTCGTATGCCGT2090.23611016968300233Illumina PCR Primer Index 7 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGCCGCCAATTCATCTCGTATGCCGT1850.20899704015002596No Hit
CTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCCGTC1820.20560789895840395No Hit
CTTAAACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCCGT1670.18866219300029374No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGAATGCCGT1520.1717164870421835No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTA1480.16719763212002078No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTAAGCCGT1410.15928963600623602No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCAATTAATCTCGTATGCCGT1350.15251135362299195No Hit
CTTATACACATCTCCGAGCCCACGAGACGACAATTCATCTCGTATGCCGT1150.12991707901217833No Hit
TATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCCGTCT1140.12878736528163764RNA PCR Primer, Index 45 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGCCAATTCCTCTCGTATGCCGT1110.1253982240900156No Hit
CTTATACACATCTCCGAGACCACGAGACGCCAATTCATCTCGTATGCCGT1090.12313879662893422No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCAATACATCTCGTATGCCGT1000.1129713730540681No Hit
CCTTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCCG960.10845251813190537No Hit
ATTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCCGT900.1016742357486613No Hit
CTTTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCCG900.1016742357486613No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCAGAA150.002218106870.030
CGGCATG150.002218106870.037
GTGGGCC150.002218106870.021
CGCCTAT150.002218106870.028
ATCCGAG207.888315E-570.012
AAAAACG401.3278623E-1070.066
AAACGGG353.6616257E-970.068
ATATCCG207.888315E-570.010
TGCGCGT150.002218106870.016
AAGCATG207.888315E-570.054
CTCGGCT150.002218106870.062
CGCTACT150.002218106870.025
GCCTATT150.002218106870.029
GCGATAA150.002218106870.019
CGTCATA207.888315E-570.048
GTGCGCG150.002218106870.015
AGGGGGG23150.065.1619970
TCGAATG650.064.6153940
AGCCGTC550.063.6363645
AAGCCGT550.063.6363644
Produced by FastQC (version 0.11.5)