FastQCFastQC Report
Wed 25 May 2016
SRR1780886_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780886_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences339747
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG323099.509723411832923No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGC150.002224757369.9904315
GCCGTAT707.2759576E-1249.99316854
TAACGGC305.864698E-446.680936
CAGGGCA305.877482E-446.6602911
CTCGCTA451.6838458E-646.6602913
TGGTCGC751.4551915E-1146.66028649
CGGTGGT802.910383E-1143.74402246
AGGGCAC350.001255363839.99453412
CTCGGTG801.4206307E-939.3696244
TCGCCGT901.05501385E-1038.8835752
CGCGAGA556.6780485E-638.18784332
GCTCGCG556.6780485E-638.18784329
ATTAGAG753.5339326E-837.3282283
GTCGCCG951.8735591E-1036.8370751
TCGGTGG951.8735591E-1036.8370745
TACGTGA501.7048925E-435.01067736
TTTAACG400.00241517235.00552434
ACGGCCG400.002416917435.0003738
CGCGGTA400.002418663634.99521643
CGGTACC400.002418663634.99521645
Produced by FastQC (version 0.11.5)