FastQCFastQC Report
Wed 25 May 2016
SRR1780886_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780886_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences339747
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAATGTCTATCTCGTATGCCGT211906.236993998475336No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAATGTCTATCTCGTATGCCG35611.0481328753454775No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAATGTCTATCTCGTATGCC20870.6142806264661645No Hit
ATACACATCTCCGAGCCCACGAGACTAATGTCTATCTCGTATGCCGTCTT7080.20839036106279082RNA PCR Primer, Index 15 (95% over 23bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5640.16600588084662998No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTAATGTCTATCTCGTATGCC5360.15776445413793205No Hit
CTTATACACATCTCCGCGCCCACGAGACTAATGTCTATCTCGTATGCCGT4340.1277421139848181No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTCGT401.0631993E-861.25747337
TAACTCG401.0631993E-861.25747336
AGGGGGG24150.059.71743470
GATACAA200.00693236652.529611
TTAATAC200.00693640652.5218733
CGTATGC33850.052.11861841
CGTCTTC32300.052.1117648
CCGTCTT32100.052.10939447
GCCGTCT32600.052.06157346
CTTGAAA32950.051.9563257
TATGCCG33450.051.8894143
TCGTATG34700.051.85070440
CTCGTAT34150.051.66076739
GTATGCC33750.051.53185742
TCTCGTA34400.051.4888438
TCTTCTG32900.051.48048450
CTGCTTG32600.051.33283254
TGCCGTC33550.051.21322645
ATCTCGT34250.051.20332737
ATGCCGT33650.051.16502844