FastQCFastQC Report
Wed 25 May 2016
SRR1780885_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780885_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences362174
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG318418.79163054222556No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATCT200.00694891652.49865339
GCCGTAA200.00694891652.4986531
TCGCCGT950.051.5847552
GCCGTAT950.047.90012454
TGGTCGC1150.045.65100549
CTCGGTG1100.044.5443144
GATCTCG1050.043.33222241
TCGGTGG1150.042.60760545
CGGTGGT1250.041.99892446
GTCGCCG1200.040.83228751
CGCGTCT350.001254788639.9989747
TGCCGGC701.9224899E-839.99897419
ATCTCGG1200.037.91569542
GTGGTCG1500.037.3323848
CGTATCA1350.036.30037756
TCTCGGT1350.036.29536443
CCATAGA501.7084328E-434.9991042
CAGTAGT400.00241755734.9991041
AGATCTC1350.033.7028440
CGCCGTA1450.031.3828453
Produced by FastQC (version 0.11.5)