FastQCFastQC Report
Wed 25 May 2016
SRR1780885_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780885_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences362174
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCCGAGATATCTCGTATGCCGT189255.225388901467251No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCCGAGATATCTCGTATGCCG31880.8802398846963062No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCCGAGATATCTCGTATGCC13240.365570140319294No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5580.15406959085964206No Hit
ATACACATCTCCGAGCCCACGAGACCCCGAGATATCTCGTATGCCGTCTT5320.1468907210346408RNA PCR Primer, Index 31 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACACCGAGATATCTCGTATGCCGT4640.12811521533848372No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCCCGAGATATCTCGTATGCC4290.11845135211252049No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG24150.059.5730970
CGACTTC308.368848E-658.3329948
AATGGTC200.00694837452.4996943
GCGTCTA200.00694837452.499693
TAATCCG200.00694837452.4996952
AGCCGTC608.367351E-1152.49968745
CCGTCTT31600.051.39209747
CGTCTTC31900.051.34766448
AACCCGA750.051.3330327
GCCGTCT32300.051.14520646
TATGCCG33850.050.7677943
TCGTATG35400.050.522340
CGTATGC34350.050.4363841
TCTTCTG30900.050.40423250
ATCTCGT35750.050.3213837
GTCTTCT31150.050.2244249
TGCCGTC33900.050.17669345
CTCGTAT35300.050.16967839
TCTCGTA35700.050.0977438
GTATGCC34600.049.86964842