FastQCFastQC Report
Wed 25 May 2016
SRR1780884_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780884_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences290624
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3360111.561674190706892No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTTGG207.900351E-570.0327151
GATCTAT207.900351E-570.0327151
CGACGAG150.00222517969.9845122
GCTTGGG200.00693400652.5245361
CAAGCGG200.006943457352.50645413
CACCGAA200.006943457352.50645412
GCGCTGG305.868552E-446.67240515
CTCGTGG350.001253462440.00491764
GGTATGA400.002410933835.0163571
GTTGTAA501.7078381E-434.9982818
TCCGTAC400.002417051734.9982818
GGCGCGT400.002417051734.998285
CGTTAAT400.002419093634.99225624
TTGACAC400.002419093634.99225635
GTATGGA552.9858356E-431.8220943
CAGTCAC552.9858356E-431.82209457
CGGTGGT1001.2540113E-831.49845146
GACCGCG450.00430062531.11493513
GCTGGCT450.004304250231.10958119
TACGGTC450.004304250231.10958110
Produced by FastQC (version 0.11.5)