FastQCFastQC Report
Wed 25 May 2016
SRR1780884_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780884_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences290624
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCGT210547.244412023783307RNA PCR Primer, Index 14 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCG34251.178498678705131No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCC18480.6358731556925787RNA PCR Primer, Index 14 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCGTCTT7140.2456782646994054RNA PCR Primer, Index 14 (96% over 26bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5890.2026673640167364No Hit
CTTATACACATCTCCGCGCCCACGAGACACAGTTGCATCTCGTATGCCGT5640.1940651838802026RNA PCR Primer, Index 14 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCC5550.19096839903105042RNA PCR Primer, Index 14 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGACGT3610.12421548117154811No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCTAT207.913457E-570.009291
GACGACT150.002223573469.99724646
AGGGAAT150.002223573469.99724617
GCCAAGT150.002223573469.99724618
ATTCGCA150.00222509269.9852129
CGAATGC550.063.62291341
TGCCGTA401.063745E-861.2475945
CGTAAGA401.0652002E-861.2370541
CGTCTTA750.060.67472548
AGGGGGG24950.060.3286570
ATCGTAT900.058.3310439
GCCGTAT308.366353E-658.3310446
AACTCGT553.6379788E-1157.27047737
CGGGTCC252.3905502E-455.988167
ACGTATG653.6379788E-1253.8347740
CTTTATA1450.053.0830882
GTATTCT407.457984E-752.5069749
CGTCTAC407.457984E-752.5069748
ATCTTCG200.00694318852.5069726
CACGTAT608.367351E-1152.49793639
Produced by FastQC (version 0.11.5)