FastQCFastQC Report
Wed 25 May 2016
SRR1780883_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780883_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152006
Sequences flagged as poor quality0
Sequence length76
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3231621.25968711761378No Hit
CTTATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTG3150.20722866202649895No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGT252.830564E-669.994085
GCGCGTC252.3854163E-455.9952626
CATACCG200.006933785552.51283357
GGCGTCA200.006942816552.4955620
TTGTACC200.006942816552.495566
CTTTACC200.006942816552.4955632
CGCGTCT200.006942816552.495567
GTGCGCG407.4454147E-752.495564
TGTGCGC451.6770609E-646.6627163
AGCGGGC350.001252855439.99661610
TGTTCTC350.001252855439.99661619
TTGTGCG459.168835E-538.8855972
GCGTCTG459.168835E-538.8855978
CGGTGGT1007.2759576E-1238.49674246
CGTATCA708.617535E-735.00855656
TCGGTGG1102.3646862E-1134.99704445
GGCAACC400.002413858234.9970429
CGTCTGG501.7043707E-434.997049
TTATAAG400.002413858234.9970428
TCGCGAG400.002413858234.9970432
Produced by FastQC (version 0.11.5)