##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780883_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 152006 Sequences flagged as poor quality 0 Sequence length 76 %GC 60 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.03806428693604 32.0 27.0 32.0 13.0 32.0 2 28.976283830901412 32.0 32.0 32.0 13.0 32.0 3 28.735418338749785 32.0 27.0 32.0 13.0 32.0 4 28.108897017223004 32.0 27.0 32.0 13.0 32.0 5 28.70674183913793 32.0 32.0 32.0 13.0 32.0 6 32.6013775772009 37.0 32.0 37.0 13.0 37.0 7 32.25318079549491 37.0 32.0 37.0 13.0 37.0 8 32.56067523650382 37.0 32.0 37.0 13.0 37.0 9 32.54088654395221 37.0 32.0 37.0 13.0 37.0 10-11 32.3070207754957 37.0 32.0 37.0 13.0 37.0 12-13 32.1691051669013 37.0 32.0 37.0 13.0 37.0 14-15 32.950692735813064 37.0 34.5 37.0 17.5 37.0 16-17 32.92958830572478 37.0 34.5 37.0 17.5 37.0 18-19 32.30905030064603 37.0 32.0 37.0 13.0 37.0 20-21 32.80388932015842 37.0 34.5 37.0 17.5 37.0 22-23 32.72736273568149 37.0 34.5 37.0 17.5 37.0 24-25 32.296123179348186 37.0 32.0 37.0 13.0 37.0 26-27 32.76760127889688 37.0 34.5 37.0 17.5 37.0 28-29 32.57300698656632 37.0 32.0 37.0 13.0 37.0 30-31 32.41921700459192 37.0 32.0 37.0 13.0 37.0 32-33 32.56326066076339 37.0 32.0 37.0 13.0 37.0 34-35 32.43436443298291 37.0 32.0 37.0 13.0 37.0 36-37 33.015173743141716 37.0 34.5 37.0 17.5 37.0 38-39 33.053261055484654 37.0 34.5 37.0 17.5 37.0 40-41 32.56603028827809 37.0 32.0 37.0 13.0 37.0 42-43 32.39915200715761 37.0 32.0 37.0 13.0 37.0 44-45 32.07921068905175 37.0 32.0 37.0 13.0 37.0 46-47 32.38459666065813 37.0 32.0 37.0 13.0 37.0 48-49 32.88561306790521 37.0 34.5 37.0 13.0 37.0 50-51 33.15034274962831 37.0 34.5 37.0 13.0 37.0 52-53 32.1901470994566 37.0 32.0 37.0 13.0 37.0 54-55 32.318967672328725 37.0 32.0 37.0 13.0 37.0 56-57 32.09666065813192 37.0 32.0 37.0 13.0 37.0 58-59 31.716195413338948 37.0 32.0 37.0 13.0 37.0 60-61 31.44041353630778 37.0 29.5 37.0 13.0 37.0 62-63 31.228783732221096 37.0 29.5 37.0 13.0 37.0 64-65 31.149352657131956 37.0 29.5 37.0 13.0 37.0 66-67 31.148744128521244 37.0 27.0 37.0 13.0 37.0 68-69 31.28930436956436 37.0 27.0 37.0 13.0 37.0 70-71 31.377948896754077 37.0 32.0 37.0 13.0 37.0 72-73 31.684038787942583 37.0 32.0 37.0 13.0 37.0 74-75 32.035090720103156 37.0 32.0 37.0 13.0 37.0 76 31.81012591608226 37.0 32.0 37.0 13.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 11101 1 1.0753618208519171 11101 2 1.1540999703628714 11101 3 0.7361334232333832 11101 4 -0.1758786716662435 11101 5 -0.47784947138201517 11101 6 0.6374104466564603 11101 7 0.29187749343793 11101 8 -0.3754885578643581 11101 9 0.4172295635409071 11101 10-11 0.08345253120393181 11101 12-13 0.9607939508565693 11101 14-15 0.811647359328667 11101 16-17 0.2689713953679842 11101 18-19 0.48159457659077987 11101 20-21 -0.014233558048182715 11101 22-23 0.7721226830637278 11101 24-25 1.3114408498370338 11101 26-27 0.2856052530651709 11101 28-29 0.10622686118329483 11101 30-31 0.30904576460710587 11101 32-33 0.16682831704262213 11101 34-35 0.0010660004102334142 11101 36-37 0.6304970125756029 11101 38-39 -0.15911975674389822 11101 40-41 -0.427635500926975 11101 42-43 -0.14627925709454814 11101 44-45 -0.4188389028388606 11101 46-47 0.22983986536257817 11101 48-49 -0.39707809652672665 11101 50-51 0.23812171496744128 11101 52-53 1.2330193181972149 11101 54-55 0.307490404038667 11101 56-57 -0.25612863121963514 11101 58-59 -0.5386609731253991 11101 60-61 0.7315368033333804 11101 62-63 0.7792517848467178 11101 64-65 0.26897703832538866 11101 66-67 -0.21068001991258 11101 68-69 -0.3393606167069514 11101 70-71 -0.367611400857232 11101 72-73 -0.6337579830525115 11101 74-75 -0.48513460541137476 11101 76 -1.4480210249835963 11102 1 0.07788707337716971 11102 2 0.3545418895547918 11102 3 0.32641120101115817 11102 4 -1.6058029140904857 11102 5 -1.3174959360284788 11102 6 0.19612256786858495 11102 7 0.11163759444802679 11102 8 0.9089180077922023 11102 9 -0.8368739718126328 11102 10-11 -0.7696093374829331 11102 12-13 -2.2821037764161574 11102 14-15 -0.8769574386511323 11102 16-17 -0.28216496826838267 11102 18-19 -0.5464988577526526 11102 20-21 -0.7036274974421275 11102 22-23 -0.015913933097891686 11102 24-25 -0.13836975622357528 11102 26-27 -1.3911624237025073 11102 28-29 -2.1767592499278194 11102 30-31 -1.5738456495343094 11102 32-33 -1.6526161274018207 11102 34-35 -0.25003879756956593 11102 36-37 -1.2391368258082416 11102 38-39 -1.3366765749257112 11102 40-41 -0.8111582281997016 11102 42-43 -1.3186277419430361 11102 44-45 -0.9093692058691651 11102 46-47 0.24246612798884115 11102 48-49 -0.5713205207691487 11102 50-51 -0.17112702240628863 11102 52-53 -0.451797600994702 11102 54-55 -0.19677095959769986 11102 56-57 -1.7131993382903445 11102 58-59 -0.2563061751455997 11102 60-61 -0.7795111764646059 11102 62-63 0.18218739090732683 11102 64-65 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11103 44-45 -0.06572885499197056 11103 46-47 -0.8356040474497561 11103 48-49 -1.0739520997165144 11103 50-51 -0.2501292153887462 11103 52-53 0.3296168726895061 11103 54-55 0.2508715842619509 11103 56-57 -0.5035502154833225 11103 58-59 -0.27927766637367313 11103 60-61 0.9429668937108353 11103 62-63 -1.409424451197939 11103 64-65 -0.23968856507705283 11103 66-67 0.37283938465945354 11103 68-69 -1.050950196717583 11103 70-71 -0.7960537187732335 11103 72-73 -0.25061072095788006 11103 74-75 0.05277076407294601 11103 76 0.21949625305999731 11104 1 -0.7087429632528668 11104 2 -0.2783564620935586 11104 3 0.5651932522932093 11104 4 -1.9091454049329784 11104 5 -1.6421296356621795 11104 6 -0.37690856766255365 11104 7 -0.9111417095812762 11104 8 -0.9490261314019328 11104 9 -0.13277598646464384 11104 10-11 -0.4500695023180974 11104 12-13 -0.5888230987604821 11104 14-15 -0.7555632078819059 11104 16-17 -0.49393236753578407 11104 18-19 -0.8135547185585139 11104 20-21 0.03721499340036871 11104 22-23 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11105 5 0.1005148166596932 11105 6 0.8553967614169693 11105 7 0.1993392073512581 11105 8 1.405221771233066 11105 9 0.20445667334865902 11105 10-11 -0.9142665955474492 11105 12-13 -0.48153253985702094 11105 14-15 0.21259229253166723 11105 16-17 -1.1484284091286021 11105 18-19 -0.7645768147419005 11105 20-21 0.2990606746008879 11105 22-23 -0.8279099008398241 11105 24-25 -1.0795659927827153 11105 26-27 -1.0040656495089593 11105 28-29 0.3069437070614285 11105 30-31 -0.045955864588073325 11105 32-33 -1.2829924714878445 11105 34-35 1.170828137914313 11105 36-37 -0.43369327742114194 11105 38-39 0.43130057561192103 11105 40-41 0.4418659653486827 11105 42-43 0.5785496774118002 11105 44-45 -0.3240197435035732 11105 46-47 -0.26022204405417426 11105 48-49 -0.3320732089411891 11105 50-51 -0.3594670761697287 11105 52-53 -0.3528392676613663 11105 54-55 -0.7496943466944721 11105 56-57 -0.7696509511935687 11105 58-59 -0.5094983794466792 11105 60-61 0.33776032891173813 11105 62-63 -0.30285965210342525 11105 64-65 0.17774210838078375 11105 66-67 0.5812717559101621 11105 68-69 1.024601585964934 11105 70-71 0.5162269829811557 11105 72-73 -0.6192581459720259 11105 74-75 -0.09640863604024474 11105 76 -0.6295133677662612 11106 1 -1.7966550511649544 11106 2 -0.9376971214342191 11106 3 -0.5666748795749221 11106 4 -1.1179366137241864 11106 5 -0.4223494158819605 11106 6 -2.1681173588713456 11106 7 -2.0649878634274295 11106 8 -1.7951799775557795 11106 9 -2.1437649974536583 11106 10-11 -0.8731464253950207 11106 12-13 -1.5448670548044383 11106 14-15 -0.6182005705192637 11106 16-17 -1.9170092906127039 11106 18-19 -1.6047635097673059 11106 20-21 -1.5561916000062261 11106 22-23 -0.5290206180795707 11106 24-25 -1.8715428331466555 11106 26-27 -1.8673528998929818 11106 28-29 -1.9829062279497975 11106 30-31 -2.213154257592919 11106 32-33 -1.501059350845047 11106 34-35 -1.2547892554450222 11106 36-37 0.29394467602325847 11106 38-39 -0.9553235529476893 11106 40-41 -0.4442855175769935 11106 42-43 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-0.8170136262461831 21611 68-69 -1.6609549383012734 21611 70-71 0.24595671271088193 21611 72-73 -0.29319014248466857 21611 74-75 -0.20408082435759312 21611 76 0.874119297156728 21612 1 0.26728101277111094 21612 2 -0.4448899286270276 21612 3 -0.11033122323126321 21612 4 -0.3940604898480622 21612 5 0.0817464882139447 21612 6 -0.44743803819202554 21612 7 -1.0283245267640915 21612 8 -0.3310441134199138 21612 9 0.6667245130358523 21612 10-11 0.2011292988807014 21612 12-13 0.6047333447959602 21612 14-15 0.900031197712508 21612 16-17 0.10381988021646293 21612 18-19 -0.8951731001768977 21612 20-21 0.12213007831545042 21612 22-23 0.5089913699324171 21612 24-25 0.27810751650370236 21612 26-27 0.17550424296415912 21612 28-29 0.40420665916308707 21612 30-31 -0.25661080104946166 21612 32-33 -0.22004036982606578 21612 34-35 0.6046013539455828 21612 36-37 -0.018492886414300358 21612 38-39 0.6136075159833823 21612 40-41 0.6026675293760562 21612 42-43 0.14311468229938384 21612 44-45 -0.47338435738431883 21612 46-47 0.35004188556460036 21612 48-49 0.30140675195812605 21612 50-51 0.497717674563404 21612 52-53 -1.2225362373583408 21612 54-55 1.00900555555382 21612 56-57 1.2610430859520818 21612 58-59 -0.24674178120620738 21612 60-61 0.08052670232327586 21612 62-63 0.5807669363618722 21612 64-65 0.11796693731528762 21612 66-67 -0.5056295148620755 21612 68-69 0.040437363091029965 21612 70-71 -0.9080154412612735 21612 72-73 0.5824036331091023 21612 74-75 0.2805219602451885 21612 76 0.22571634875377455 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 1.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 1.0 11 19.0 12 44.0 13 116.0 14 173.0 15 332.0 16 489.0 17 667.0 18 894.0 19 1084.0 20 1351.0 21 1822.0 22 2283.0 23 2743.0 24 3181.0 25 3600.0 26 4273.0 27 4607.0 28 5313.0 29 5979.0 30 6891.0 31 8469.0 32 10964.0 33 14734.0 34 21958.0 35 29587.0 36 20431.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 59.731442067935546 14.339098798002592 6.944216004263241 18.985243129798615 2 42.60225396219712 18.66854387199916 21.553431272574162 17.17577089322956 3 39.84696260913619 18.630953555849437 23.043114962267005 18.47896887274737 4 36.73635873212194 15.857686083077855 25.374008236733726 22.031946948066473 5 36.09994539581456 24.300178285954882 26.434346690525846 13.165529627704716 6 46.61679857369359 27.509687436266866 14.637403701291438 11.236110288748101 7 46.17302631578947 23.436184210526314 15.398684210526314 14.992105263157896 8 44.70694271748212 25.707725607068376 15.156479233689252 14.42885244176025 9 43.23298377652925 16.374784542308653 15.030723279956842 25.361508401205246 10-11 44.35460526315789 19.858223684210525 19.623355263157894 16.163815789473684 12-13 43.91373570704878 19.356504690851196 18.896630218818668 17.83312938328136 14-15 43.53276709408738 19.108600827647916 17.35264511799575 20.005986960268952 16-17 43.16293299121682 21.35958419684858 17.417349254909702 18.0601335570249 18-19 41.753784614170016 21.828720303690204 19.1878787081324 17.22961637400738 20-21 42.57847836026855 21.40387696093105 19.02921371969171 16.988430959108687 22-23 42.78792384310733 20.94314548492783 18.68363574163498 17.585294930329866 24-25 42.429784798384176 21.70273097495345 18.398389441896878 17.46909478476549 26-27 42.7037624257416 20.962086274613 19.329552706196587 17.004598593448815 28-29 42.810198449377815 20.685707894884 19.71796702049624 16.786126635241953 30-31 42.82556494852143 20.7706983322917 19.196736949442453 17.206999769744417 32-33 42.4676324307255 21.257006394568563 19.747835583274124 16.527525591431804 34-35 42.732284656022 21.133084650758878 19.099874342931955 17.034756350287168 36-37 43.224199920395 20.380525064062287 19.89203982881635 16.503235186726357 38-39 43.05390167040572 20.164935295626947 20.44421344877269 16.336949585194642 40-41 42.79807369688357 20.089670462694325 20.192959256846997 16.919296583575107 42-43 42.20184995131707 20.181705744585667 20.49584221467856 17.1206020894187 44-45 41.62889229534405 19.835656813531667 20.827439654208252 17.708011236916033 46-47 41.72982539243937 19.52427005618495 20.813760707377728 17.932143843997945 48-49 41.52176630416905 19.20020263024585 22.087354688455996 17.190676377129098 50-51 41.466527630206876 19.27551916630756 21.54955476534308 17.70839843814248 52-53 40.14631675208389 20.442568701109877 21.306719123152128 18.104395423654104 54-55 40.21065512295891 20.624399678951594 20.71781950237497 18.44712569571453 56-57 40.440475015625516 20.514819566433108 20.518109148327248 18.52659626961413 58-59 40.35870308963629 20.441536396652456 21.06459550502658 18.135165008684666 60-61 40.19749740134998 20.47900685517296 20.44775726635176 18.8757384771253 62-63 40.44736842105263 20.085197368421053 20.573355263157893 18.89407894736842 64-65 40.892972275951 20.095988105106645 20.317043645311124 18.693995973631232 66-67 41.02770751027148 20.15644891232479 19.19755787063688 19.618285706766844 68-69 41.10311387724758 19.533609215971367 19.302025697875617 20.06125120890544 70-71 41.199421014540434 19.683202842292253 19.087110994144354 20.030265149022963 72-73 41.81918658965014 19.16060314995855 18.61883396271102 20.401376297680294 74-75 41.57535846214068 18.744963109402086 19.061732131168032 20.61794629728921 76 42.07098450708858 18.441498634913327 18.69741126936614 20.790105588631953 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 1.5 7 1.5 8 0.0 9 1.0 10 4.0 11 6.5 12 7.0 13 12.5 14 21.5 15 33.5 16 52.0 17 62.0 18 76.5 19 119.0 20 147.0 21 147.0 22 186.0 23 287.0 24 381.5 25 414.0 26 482.0 27 665.5 28 926.5 29 1072.0 30 1153.0 31 1449.0 32 1854.0 33 2044.0 34 2326.5 35 2846.0 36 3329.5 37 3576.0 38 3953.0 39 4706.5 40 5448.0 41 5976.5 42 6140.0 43 6463.0 44 6966.5 45 7056.0 46 6965.0 47 7045.0 48 6819.0 49 6228.5 50 5944.0 51 5454.0 52 4755.5 53 4144.0 54 3741.0 55 3497.0 56 2917.5 57 2381.0 58 2180.0 59 1927.0 60 1505.5 61 1250.5 62 1164.0 63 1067.5 64 923.0 65 822.0 66 705.5 67 642.0 68 607.5 69 527.0 70 459.5 71 438.0 72 415.0 73 380.0 74 352.5 75 337.0 76 289.5 77 255.0 78 266.5 79 265.0 80 252.0 81 232.0 82 219.5 83 214.0 84 196.0 85 180.0 86 183.0 87 184.0 88 202.0 89 218.5 90 244.0 91 295.0 92 319.0 93 340.5 94 434.5 95 587.5 96 668.0 97 870.5 98 1610.0 99 17543.5 100 32940.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.005920818915042827 2 0.004605081378366643 3 0.011183769061747562 4 0.0026314750733523675 5 0.001973606305014276 6 0.0032893438416904596 7 0.003947212610028552 8 0.0032893438416904596 9 0.0026314750733523675 10-11 0.003947212610028552 12-13 0.005262950146704735 14-15 0.005920818915042827 16-17 0.007236556451719012 18-19 0.005920818915042827 20-21 0.003618278225859506 22-23 0.0026314750733523675 24-25 0.005920818915042827 26-27 0.001973606305014276 28-29 0.0016446719208452298 30-31 6.578687683380919E-4 32-33 0.0013157375366761837 34-35 0.0032893438416904596 36-37 0.003618278225859506 38-39 0.004605081378366643 40-41 0.0032893438416904596 42-43 0.0013157375366761837 44-45 0.004605081378366643 46-47 0.005262950146704735 48-49 0.0032893438416904596 50-51 0.004934015762535689 52-53 0.004605081378366643 54-55 0.0026314750733523675 56-57 0.007236556451719012 58-59 0.009210162756733286 60-61 0.0026314750733523675 62-63 0.003947212610028552 64-65 0.005262950146704735 66-67 0.004934015762535689 68-69 0.005920818915042827 70-71 0.01052590029340947 72-73 0.0026314750733523675 74-75 0.0016446719208452298 76 6.578687683380919E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 152006.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.78306119495283 #Duplication Level Percentage of deduplicated Percentage of total 1 75.98896440733982 34.790074076023316 2 7.934706076760594 7.265502677525887 3 5.741956806000603 7.886530794837046 4 3.879700544595002 7.104982698051393 5 2.467202161136896 5.647803376182519 6 1.4469846105211732 3.9748430982987513 7 0.8391648585346227 2.6893675249661197 8 0.4856810311381892 1.7788771495862006 9 0.30031756067420573 1.237451153243951 >10 0.902389608150245 5.803718274278647 >50 0.008621556765766673 0.25591095088351773 >100 0.0028738522552555572 0.30525110850887466 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0014369261276277786 21.25968711761378 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 32316 21.25968711761378 No Hit CTTATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTG 315 0.20722866202649895 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.005262950146704735 0.0 2 0.0 0.0 0.0 0.005262950146704735 0.0 3 0.0 0.0 0.0 0.005262950146704735 0.0 4 0.0 0.0 0.0 0.009868031525071378 0.0 5 0.0 0.0 0.0 0.015130981671776114 0.0 6 0.0 0.0 0.0 0.015130981671776114 0.0 7 0.0 0.0 0.0 0.01710458797679039 0.0 8 0.0 0.0 0.0 0.01710458797679039 0.0 9 0.0 0.0 0.0 0.023025406891833217 0.0 10 0.0 0.0 0.0 0.024999013196847494 0.0 11 0.0 0.0 0.0 0.028288357038537954 0.0 12 0.0 0.0 0.0 0.0328934384169046 0.0 13 0.0 0.0 0.0 0.0328934384169046 0.0 14 0.0 0.0 0.0 0.036840651026933145 0.0 15 0.0 0.0 0.0 0.04210360117363788 0.0 16 0.0 0.0 0.0 0.04276146994197597 0.0 17 0.0 0.0 0.0 0.04276146994197597 0.0 18 0.0 0.0 0.0 0.04276146994197597 0.0 19 0.0 0.0 0.0 0.05131376393037117 0.0 20 0.0 0.0 0.0 0.052629501467047354 0.0 21 0.0 0.0 0.0 0.0657868768338092 0.0 22 0.0 0.0 0.0 0.07170769574885202 0.0 23 0.0 0.0 0.0 0.07499703959054248 0.0 24 0.0 0.0 0.0 0.08683867742062813 0.0 25 0.0 0.0 0.0 0.09670670894569951 0.0 26 0.0 0.0 0.0 0.1065747404707709 0.0 27 0.0 0.0 0.0 0.12433719721589938 0.0 28 0.0 0.0 0.0 0.1467047353393945 0.0 29 0.0 0.0 0.0 0.18617686143968 0.0 30 0.0 0.0 0.0 0.24867439443179876 0.0 31 0.0 0.0 0.0 0.32169782771732697 0.0 32 0.0 0.0 0.0 0.36972224780600765 0.0 33 0.0 0.0 0.0 0.4585345315316501 0.0 34 0.0 0.0 0.0 0.5499782903306448 0.0 35 0.0 0.0 0.0 0.6822099127666014 0.0 36 0.0 0.0 0.0 0.9197005381366525 0.0 37 0.0 0.0 0.0 1.3006065550044077 0.0 38 0.0 0.0 0.0 1.745325842400958 0.0 39 0.0 0.0 0.0 2.23412233727616 0.0 40 0.0 0.0 0.0 2.9248845440311566 0.0 41 0.0 0.0 0.0 3.6413036327513386 0.0 42 0.0 0.0 0.0 4.428114679683697 0.0 43 0.0 0.0 0.0 5.145849505940555 0.0 44 0.0 0.0 0.0 6.0267357867452604 0.0 45 0.0 0.0 0.0 6.909595673854979 0.0 46 0.0 0.0 0.0 7.779956054366275 0.0 47 0.0 0.0 0.0 8.579924476665395 0.0 48 0.0 0.0 0.0 9.245687670223544 0.0 49 0.0 0.0 0.0 9.918687420233411 0.0 50 0.0 0.0 0.0 10.619317658513479 0.0 51 0.0 0.0 0.0 11.337710353538677 0.0 52 0.0 0.0 0.0 12.006762890938516 0.0 53 0.0 0.0 0.0 12.643579858689789 0.0 54 0.0 0.0 0.0 13.384997960606817 0.0 55 0.0 0.0 0.0 14.028393616041472 0.0 56 0.0 0.0 0.0 14.663236977487731 0.0 57 0.0 0.0 0.0 15.34281541518098 0.0 58 0.0 0.0 0.0 15.905951080878387 0.0 59 0.0 0.0 0.0 16.41777298264542 0.0 60 0.0 0.0 0.0 16.9631461915977 0.0 61 0.0 0.0 0.0 17.51180874439167 0.0 62 0.0 0.0 0.0 18.131521124166152 0.0 63 0.0 0.0 0.0 18.781495467284188 0.0 64 0.0 0.0 0.0 19.33936818283489 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCGCGT 25 2.830564E-6 69.99408 5 GCGCGTC 25 2.3854163E-4 55.995262 6 CATACCG 20 0.0069337855 52.512833 57 GGCGTCA 20 0.0069428165 52.49556 20 TTGTACC 20 0.0069428165 52.49556 6 CTTTACC 20 0.0069428165 52.49556 32 CGCGTCT 20 0.0069428165 52.49556 7 GTGCGCG 40 7.4454147E-7 52.49556 4 TGTGCGC 45 1.6770609E-6 46.662716 3 AGCGGGC 35 0.0012528554 39.996616 10 TGTTCTC 35 0.0012528554 39.996616 19 TTGTGCG 45 9.168835E-5 38.885597 2 GCGTCTG 45 9.168835E-5 38.885597 8 CGGTGGT 100 7.2759576E-12 38.496742 46 CGTATCA 70 8.617535E-7 35.008556 56 TCGGTGG 110 2.3646862E-11 34.997044 45 GGCAACC 40 0.0024138582 34.99704 29 CGTCTGG 50 1.7043707E-4 34.99704 9 TTATAAG 40 0.0024138582 34.99704 28 TCGCGAG 40 0.0024138582 34.99704 32 >>END_MODULE