FastQCFastQC Report
Wed 25 May 2016
SRR1780883_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780883_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152006
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATTTATCTATCTCGTATGCCGT2137614.062602791995053No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTTATCTATCTCGTATGCCG37782.485428206781311No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATTTATCTATCTCGTATGCC18851.2400826283173032No Hit
ATACACATCTCCGAGCCCACGAGACATTTATCTATCTCGTATGCCGTCTT7190.473007644435088RNA PCR Primer, Index 38 (95% over 22bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6430.42300961804139314No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATTTATCTATCTCGTATGCC6340.4170887991263503No Hit
CTTATACACATCTCCGCGCCCACGAGACATTTATCTATCTCGTATGCCGT3940.2592002947252082No Hit
CTTATACACATCTCCGAGCCCACGAGACATTTATCTATCTCGTATGACGT2940.19341341789139904No Hit
CTTATACACATCTCCGAGCCCACGAGACATTTATCTATCTCGTATGCAGT2240.14736260410773258No Hit
CTATACACATCTCCGAGCCCACGAGACATTTATCTATCTCGTATGCCGTC2080.1368367038143231No Hit
CTTATACACATCTCCGAGCCCACGAGACATATATCTATCTCGTATGCCGT1940.12762654105758983No Hit
CTTATACACATCTCCGAGCCCACGAGACAATTATCTATCTCGTATGCCGT1900.12499506598423746No Hit
CTTATACACATCTCCGAGCCCACGAGACATTTATCTATATCGTATGCCGT1820.11973211583753272No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTGT150.002221223469.9996735
AGCCGTC150.002221223469.9996745
AATTTGG150.002221223469.9996716
ACGTGTG150.002221223469.9996736
GGAACGT150.002221223469.9996733
TACTCGC150.002221223469.9996746
CGTGTGG150.002221223469.9996737
CTTTATA900.066.11082
AGGGGGG23650.062.3041570
ATATCGT900.062.2219337
AACTCGT401.0590156E-861.24971837
ATCGTAT1000.059.49971839
TATCGTA950.058.94709438
CCGACTT601.8189894E-1258.33305747
CGTATGA800.056.87473741
TGACGTC900.054.44418745
CGACTTC653.6379788E-1253.845948
CTATGGC200.00694062352.4997563
GAGGAAC200.00694062352.49975611
TCGCAGT200.00694062352.4997568
Produced by FastQC (version 0.11.5)