FastQCFastQC Report
Wed 25 May 2016
SRR1780882_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780882_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63615
Sequences flagged as poor quality0
Sequence length76
%GC74

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2877645.23461447771752No Hit
CTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTG8881.3958971940580052No Hit
TCTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGT1150.18077497445571014No Hit
CTTATACACATCTGACGCTGCCGCCGATCTACTCTGTGTAGATCTCGGTG920.1446199795645681No Hit
ATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTGGTC850.133616285467264No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG730.11475280987188556No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG640.10060520317535172No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAACA150.002203980770.0897752
GACAGAT150.00221779469.979569
TATTGGG150.00221779469.9795631
CATACGA150.00221779469.9795662
ATACGAT150.00221779469.9795663
CACATCG200.006886989352.5673270
AATCAAT200.00693002952.48467336
ACCATTG200.00693002952.48467347
ACCGCAC200.00693002952.48467359
TGGCGCT200.00693002952.48467313
ATGCTTA200.00693002952.48467311
GATGAGG200.00693002952.4846732
GTATAGC200.00693002952.4846733
ACACTTA200.00693002952.4846736
CTGGCGG200.00693002952.48467318
AAGCTGT200.00693002952.48467340
CGTATCA1700.049.47512456
CGCCGTA1800.048.6734553
GCCGTAT1800.048.6734554
CCGTATC1750.048.06155455
Produced by FastQC (version 0.11.5)