SRR1780882_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1780882_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	63615
Sequences flagged as poor quality	0
Sequence length	76
%GC	50

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTGTCCGCGATCTCGTATGCCGT	19742	31.033561266996777	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGTCCGCGATCTCGTATGCCG	2731	4.293012654248212	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	1730	2.719484398333726	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGTCCGCGATCTCGTATGCC	1468	2.3076318478346303	No Hit
ATACACATCTCCGAGCCCACGAGACTGTCCGCGATCTCGTATGCCGTCTT	513	0.8064135817024287	TruSeq Adapter, Index 1 (95% over 21bp)
CATATACACATCTCCGAGCCCACGAGACTGTCCGCGATCTCGTATGCCGT	460	0.7230998978228406	No Hit
CTTATACACATCTCCGCGCCCACGAGACTGTCCGCGATCTCGTATGCCGT	417	0.655505776939401	No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTGTCCGCGATCTCGTATGCC	332	0.521889491472137	No Hit
CTAATACACATCTCCGAGCCCACGAGACTGTCCGCGATCTCGTATGCCGT	291	0.45743928318792737	No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTCCGCGCTCTCGTATGCCGT	226	0.3552621237129608	No Hit
ATTATACACATCTCCGAGCCCACGAGACTGTCCGCGATCTCGTATGCCGT	171	0.2688045272341429	No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTCAGCGATCTCGTATGCCGT	168	0.2640886583352983	No Hit
CTATACACATCTCCGAGCCCACGAGACTGTCCGCGATCTCGTATGCCGTC	140	0.22007388194608188	No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTCCGCGATCTCGTATGACGT	123	0.19335062485262908	No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTCCGCGATATCGTATGCCGT	98	0.15405171736225734	No Hit
CTTTATACACATCTCCGAGCCCACGAGACTGTCCGCGATCTCGTATGCCG	96	0.1509078047630276	No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTCCGCGATCTCGTATGCAGT	93	0.14619193586418297	No Hit
CTTATACACATCTCCGAGACCACGAGACTGTCCGCGATCTCGTATGCCGT	88	0.13833215436610863	No Hit
TCATATACACATCTCCGAGCCCACGAGACTGTCCGCGATCTCGTATGCCG	80	0.12575650396918966	No Hit
CCTTATACACATCTCCGAGCCCACGAGACTGTCCGCGATCTCGTATGCCG	78	0.1226125913699599	No Hit
CTTTACACATCTCCGAGCCCACGAGACTGTCCGCGATCTCGTATGCCGTC	74	0.11632476617150042	No Hit
CTTAAACACATCTCCGAGCCCACGAGACTGTCCGCGATCTCGTATGCCGT	70	0.11003694097304094	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGTCCGCGCTCTCGTATGCCG	66	0.10374911577458147	No Hit
CTTATACACATCTCCGAGCCAACGAGACTGTCCGCGATCTCGTATGCCGT	66	0.10374911577458147	No Hit
TCTTATACACATCTCCGCGCCCACGAGACTGTCCGCGATCTCGTATGCCG	65	0.10217715947496661	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCACGT	30	1.0056465E-7	70.03148	37
AATCTCG	15	0.0022112732	70.03148	36
CAGCTAG	15	0.0022112732	70.03148	54
CACGTAT	30	1.0056465E-7	70.03148	39
CGATCTA	15	0.0022112732	70.03148	35
CGATCAC	30	1.0056465E-7	70.03148	35
TCACGTA	30	1.0056465E-7	70.03148	38
CTCGTAA	20	7.85371E-5	70.03147	39
GATCACG	35	3.6252459E-9	70.03147	36
GCTAGAA	55	0.0	70.03147	56
TCGTAAG	20	7.85371E-5	70.03147	40
ACGTATG	35	3.6252459E-9	70.03147	40
CGAAAGT	15	0.002218189	69.97642	31
TCTTCCA	15	0.002218189	69.97642	22
TCTAGGG	15	0.002218189	69.97642	17
AAGCCGT	20	7.884409E-5	69.97642	44
TCTACGA	15	0.002218189	69.97642	11
TACGAGC	15	0.002218189	69.97642	13
GAGAACA	15	0.002218189	69.97642	16
CCGAGAT	20	7.884409E-5	69.97642	32

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content