FastQCFastQC Report
Wed 25 May 2016
SRR1780881_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780881_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences200280
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3128215.6191332135011No Hit
CTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTG11480.5731975234671459No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG150.002222843269.99522
AGCGCTC150.002222843269.99520
AAATTCG150.002222843269.99545
GGTTACA200.006931941552.5224881
GCTACCG200.00693879652.5093762
GTTTCGT200.006945655752.49625430
ACGATCC200.006945655752.49625424
TCTCGGT2750.047.08754743
TGGTCGC2750.045.8149149
CGGTGGT3000.045.49675446
GTGGTCG2850.045.43535648
TCGCCGT2650.044.91367352
GCCGTAT2650.044.91367354
GTCGCCG2850.044.2073751
CTCGGTG2950.043.89517644
TCGGTGG3050.043.60344345
GATCTCG2850.042.9793941
GGTGGTC3100.042.90016647
ATCTCGG2900.042.23836542
TAGATCT2900.042.22781839
Produced by FastQC (version 0.11.5)