FastQCFastQC Report
Wed 25 May 2016
SRR1780881_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780881_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences200280
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACGTGACGATCTCGTATGCCGT2051610.2436588775714No Hit
TCTTATACACATCTCCGAGCCCACGAGACACGTGACGATCTCGTATGCCG28051.4005392450569203No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21671.0819852206910325No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACGTGACGATCTCGTATGCC19310.9641501897343718No Hit
ATACACATCTCCGAGCCCACGAGACACGTGACGATCTCGTATGCCGTCTT5870.29308967445576195Illumina PCR Primer Index 1 (95% over 22bp)
CATATACACATCTCCGAGCCCACGAGACACGTGACGATCTCGTATGCCGT5490.274116237267825No Hit
CTTATACACATCTCCGCGCCCACGAGACACGTGACGATCTCGTATGCCGT4700.23467145995606153No Hit
CTTATACACATCTCCGAGCCCACGAGACACGTGACGCTCTCGTATGCCGT3380.1687637307769123No Hit
TCTTTATACACATCTCCGAGCCCACGAGACACGTGACGATCTCGTATGCC3260.16277211903335329No Hit
CTAATACACATCTCCGAGCCCACGAGACACGTGACGATCTCGTATGCCGT3110.15528260435390454No Hit
CTTATACACATCTCCGAGCCCACGAGACACGTGCCGATCTCGTATGCCGT2820.14080287597363692No Hit
CTTATACACATCTCCGAGCCCACGAGACACGTGACGATATCGTATGCCGT2060.10285600159776313No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTA207.9077676E-570.0087448
AACTCGT207.9077676E-570.0087437
AAACGTG150.002221114570.00873627
GAAACGT150.002221114570.00873626
ACGAACT150.002223315969.9912634
AGGGGGG28100.062.2853570
CCGACTT401.0622898E-861.24235547
CGTATGA1000.059.50743541
GAAGATC308.361139E-658.32604633
CGAACTC252.3847821E-456.00699635
GGTCGGT252.3847821E-456.00699611
CGTCTAC252.3847821E-456.00699648
GTCTTCA750.056.00699249
GCCGACT509.767973E-1055.9930146
CACGTGC950.055.2700628
GTGCCGA900.054.43764531
TGCCGAC452.6911948E-854.43764545
CCGTCTT35750.052.95842447
TCGTATG37100.052.9311440
CGTCTTC36300.052.84406348
Produced by FastQC (version 0.11.5)