FastQCFastQC Report
Wed 25 May 2016
SRR1780880_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780880_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences195021
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3049215.635239281923486No Hit
CTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTG12850.6589034001466509No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGAAC150.002218220470.0310515
CAGATCC150.00222500369.9771730
CAGATAC200.00694533652.49634622
GTAACTC200.00694533652.49634624
ACGACTG200.00694533652.49634645
CGGGAAC200.00695238552.4828875
TGGTCGC2850.051.5621349
GCCGTAT3000.051.35610254
TCGCCGT3050.050.51420252
CGGTGGT2900.049.47931746
GTCGCCG3050.049.34082851
TCGGTGG3000.048.9965945
ATCTCGG2900.048.29727642
CTCGGTG2950.047.47867644
CGTATCA3350.047.03577856
TCTCGGT3000.046.68736643
GATCTCG3000.046.68736641
CTACGGG305.8619265E-446.6753938
AGATCCC305.876773E-446.6514531
AGATCTC3100.046.29897340
Produced by FastQC (version 0.11.5)