FastQCFastQC Report
Wed 25 May 2016
SRR1780880_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780880_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences195021
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTCTACCAATCTCGTATGCCGT1970510.104040077735219No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCTACCAATCTCGTATGCCG28771.4752257449197779No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22981.1783346408848279No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCTACCAATCTCGTATGCC19841.0173263392147514No Hit
ATACACATCTCCGAGCCCACGAGACTTCTACCAATCTCGTATGCCGTCTT6710.34406551089369863Illumina PCR Primer Index 4 (95% over 22bp)
CTTATACACATCTCCGCGCCCACGAGACTTCTACCAATCTCGTATGCCGT4490.23023161608237064No Hit
CATATACACATCTCCGAGCCCACGAGACTTCTACCAATCTCGTATGCCGT3920.20100399444162423No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTCTACCAATCTCGTATGCC3860.1979274026899667No Hit
CTAATACACATCTCCGAGCCCACGAGACTTCTACCAATCTCGTATGCCGT2500.12819132298572974No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCTACCACTCTCGTATGCCGT2340.11998707831464303No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACAC150.002217027870.0405451
GTTCACG150.002223806769.9866743
GGTTCAC150.002223806769.9866742
AGGTCCA150.002223806769.9866710
ATATCCG252.8353552E-669.98666410
AAGCCGT401.0626536E-861.2383344
AGGGGGG25350.060.23210570
AGCCGTC407.4618583E-752.48999845
GATACAC200.0069486652.4899981
GCACGAT200.0069486652.4899987
GACCACG407.4618583E-752.48999818
AGCGGAA200.0069486652.48999818
ACGATCT200.0069486652.4899989
GCTAGTG200.0069486652.4899983
GCCGTCA407.4618583E-752.48999846
AATCTCG33000.050.1571136
CGTCTTA352.0770742E-550.0161348
ATCTCGT33100.050.00557737
AAACTCG352.0833893E-549.9904836
AACTCGT352.0833893E-549.9904837
Produced by FastQC (version 0.11.5)