FastQCFastQC Report
Wed 25 May 2016
SRR1780879_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780879_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences285211
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2952810.35303687445435No Hit
CTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTG11280.3954966673795891No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGTT200.006941749252.5094734
TGCGCGT200.006946565652.5002675
TTTTACG200.006946565652.50026729
ACGATAT200.00695138552.49106226
CGGACGA200.00695138552.49106221
ATCTCGG1800.040.83353842
GTCGCCG2150.040.69074251
GCCGTAT2000.040.25726354
TTAACAT350.001254150940.000259
GTAGATC1950.039.49430538
TCGCCGT2150.039.07681752
CACGCAA459.193727E-538.88226710
GATCTCG1900.038.68440241
CGCCGTA2200.038.18870553
TCGGTGG1850.037.83139845
AGATCTC1950.037.6991140
TGGTCGC1950.037.6858949
CTCGGTG1900.036.8422944
CGGTGGT2050.035.84755346
TAGATCT2200.035.00631339
Produced by FastQC (version 0.11.5)