FastQCFastQC Report
Wed 25 May 2016
SRR1780879_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780879_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences285211
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCGT193476.783398957263219No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCG24460.8576106812149601No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21190.7429587217884304No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCC20260.7103512837863898No Hit
ATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCGTCTT5720.20055327459319588RNA PCR Primer, Index 15 (95% over 22bp)
CTTATACACATCTCCGCGCCCACGAGACGCTTGCCAATCTCGTATGCCGT4860.1704001598816315No Hit
CATATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCGT4510.15812854342925062No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCC3590.1258717230401352No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTCGT353.6943675E-970.0159637
CGTCTAC252.8346276E-670.00368548
CGTAGAA150.002224267269.9914142
AGGGGGG27700.062.29569670
AAACTCG401.0613803E-861.26396636
AAGGGGG32350.053.00740469
CCGTCTT32850.052.94631647
CGTCTTC32800.052.92961548
TATGCCG33550.052.88471643
GCCGTCT33350.052.67719346
TCGTATG34050.052.64048840
CGTATGA608.367351E-1152.5119741
TGACGTA200.006940441752.5119739
ATCGTAT800.052.5119739
ATATCGT800.052.5119737
TACCCGC200.00694525852.5027611
CGTTACC200.00694525852.5027614
CTACCCG200.00695007752.49355710
CGTATGC34000.052.40900441
CTCGTAT33600.052.3035939