FastQCFastQC Report
Wed 25 May 2016
SRR1780878_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780878_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences180077
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3071517.056592457670884No Hit
CTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTG13360.7419048518133909No Hit
TCTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGT1990.11050828256801257No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGG150.002222549469.9944462
GTACATC200.006937119652.51041438
GAATTAC200.006937119652.51041437
TATCGCG200.006937119652.51041438
ATCGCGT200.006937119652.51041439
TCGCGTG200.006937119652.51041440
TCTCGGT2600.052.49583443
CGTGCAC200.00694474652.4958343
TACATCG200.00694474652.495838
GACATGA200.00694474652.4958333
TCGGTTC200.00694474652.4958313
TGGTCGC2600.051.14978849
GCCGTAT2550.050.7943954
CTCGGTG2650.050.18469644
TCGCCGT2700.049.26903552
GTAGATC2700.049.26903538
TCGGTGG2700.049.2553545
GATCTCG2650.048.87761741
ATCTCGG2650.048.86404842
CGGTGGT2750.048.359846
Produced by FastQC (version 0.11.5)