FastQCFastQC Report
Wed 25 May 2016
SRR1780878_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780878_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences180077
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCCGT2015711.19354498353482No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCCG31301.738145348934067No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24811.3777439650816041No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCC18461.0251170332691015No Hit
ATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCCGTCTT6210.34485247977254174TruSeq Adapter, Index 8 (95% over 21bp)
CTTATACACATCTCCGCGCCCACGAGACCATTTCGAATCTCGTATGCCGT4470.24822714727588754No Hit
CATATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCCGT3940.2187952931246078No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCC3760.20879956907322977No Hit
CTAATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCCGT2710.15049117877352466No Hit
CTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCCGTC1990.11050828256801257No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTATC150.002221954869.9991628
GCTTCGT150.002221954869.9991632
AGGGGGG23400.062.68760370
ACTCTCG252.3857466E-455.99933236
CGTATGA850.053.52877441
CTCGTAT32100.053.4261639
TCGTATG32400.053.36356440
AATCTCG32350.053.33785236
CGAATCT32700.053.19508734
TCTCGTA32650.053.1693538
ATCTCGT32500.053.09167537
GAATCTC32650.053.06215335
CGTATGC31950.053.0197141
TCGAATC33150.052.89529833
ATGCCGT31500.052.8882644
TGCCGTC31500.052.77715345
TATGCCG31650.052.74818843
CCGTCTT30400.052.72963347
CTTGAAA31000.052.72517857
GTATGCC31800.052.719542