FastQCFastQC Report
Wed 25 May 2016
SRR1780877_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780877_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences317148
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3212210.128394314326435No Hit
CTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTG11870.3742732099839823No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGAC150.002223414670.0000161
GCTATCT200.00694741952.51
GTGACCC200.00694741952.563
GCCGTAT2750.042.054
GTCGCCG2900.041.0344851
CGGTGGT2950.040.33898546
TGGTCGC2900.039.82758749
TCGCCGT3050.039.01639652
TCGGTGG3000.038.545
CGTATCA3100.038.38709656
CTCGGTG2950.037.96610344
TCTCGGT3050.036.72131343
AGATCTC3250.036.61538740
CGCCGTA3250.036.61538753
CCGTATC3350.036.56716555
GATCTCG3300.036.06060441
GTATCAT3250.035.53846457
ATCTCGG3350.035.5223942
TAGATCT3350.035.5223939
GATCATC501.7076968E-435.0000041
Produced by FastQC (version 0.11.5)