FastQCFastQC Report
Wed 25 May 2016
SRR1780877_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780877_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences317148
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTATGCCGT194496.13246812213856No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTATGCCG25520.8046716359554529No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTATGCC23160.7302584282417042No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22870.7211144323785741No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGCCCGAATCTCGTATGCCGT12270.3868856180710583No Hit
ATACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTATGCCGTCTT5470.17247468059076518RNA PCR Primer, Index 40 (95% over 21bp)
CATATACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTATGCCGT4110.12959249309470658No Hit
CTTATACACATCTCCGCGCCCACGAGACCTGACCGAATCTCGTATGCCGT3900.12297097884899164No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTATGCC3640.1147729135923922No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTGT150.002223434969.999849
AGGGGGG25700.062.373470
CCGACTT352.9691E-759.99986647
AACTCGT308.366276E-658.333237
GACCGAT252.3884152E-455.99987431
CGTCTAC252.3884152E-455.99987448
CTTGAAA31800.053.3803857
CCGTCTT31050.053.31711247
GCCGTCT31500.053.11099246
CGTCTTC30850.053.09550548
CTCGTAT34750.052.978339
TATGCCG34150.052.88421243
TCGTATG35200.052.7981840
TGCCGTC33550.052.7867745
CGTATGC34700.052.75204541
TCTCGTA35100.052.64945238
TCTTCTG30100.052.5580250
ATGCCGT34050.052.52557844
GAGTCGT200.0069474852.4998813
GCCGACT407.4671334E-752.4998846