FastQCFastQC Report
Wed 25 May 2016
SRR1780876_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780876_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences305419
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3743612.257259698970921No Hit
CTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTG9970.326436796662945No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGATCC150.00222275870.00458564
AACGTAC150.002224202369.99312624
ACGGGTG200.006945374452.5034434
GAGAGTC200.00694987452.4948436
ATCTCGC305.8754307E-446.66208325
TAGCTCG305.8754307E-446.66208310
TGGTCGC1700.039.11380449
CGGTGGT2000.038.49621646
TCGCCGT1650.038.1843252
GTCGCCG1750.037.9962751
GCGAACT654.8582297E-737.68860628
GCCGTAT1700.037.06125354
GCCCAGT400.002415885235.00229319
CCCAGTG400.002415885235.00229320
TGCCGGC501.7068919E-435.00229319
GGTCGGC400.002417826834.99656350
CAACCGT601.21444555E-534.9965636
CTCGGTG1900.034.99656344
AGCTCGT400.002417826834.99656311
CCGACCA501.7085361E-434.9965638
Produced by FastQC (version 0.11.5)