FastQCFastQC Report
Wed 25 May 2016
SRR1780876_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780876_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences305419
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGCCGTGCATCTCGTATGCCGT247768.112134477553786No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGCCGTGCATCTCGTATGCCG30701.005176495240964No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGCCGTGCATCTCGTATGCC21870.7160654707140027No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19310.6322461929349517No Hit
CTTATACACATCTCCGCGCCCACGAGACCGCCGTGCATCTCGTATGCCGT6530.21380464214734515No Hit
ATACACATCTCCGAGCCCACGAGACCGCCGTGCATCTCGTATGCCGTCTT6130.2007078799943684RNA PCR Primer, Index 16 (95% over 23bp)
CATATACACATCTCCGAGCCCACGAGACCGCCGTGCATCTCGTATGCCGT5550.18171757487255213No Hit
CTTATACACATCTCCGAGCCCACGAGCCCGCCGTGCATCTCGTATGCCGT4720.15454179340512542No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCGCCGTGCATCTCGTATGCC3690.12081763086121033No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATGG150.002223088270.0019639
GCATCGC150.002223088270.0019635
AGGGGGG32750.062.1035170
ACCGACG401.0633812E-861.2517227
ATCGTAT601.8189894E-1258.3349739
ATATCGT601.8189894E-1258.3349737
CTCGAAA252.3879168E-456.00157539
TGCCGTA750.055.992445
GACCGAC452.6942871E-854.44597626
CGTCTTA653.6379788E-1253.83885248
CGTATGC41650.053.78302441
CCGTCTT39200.053.7427147
ATCTCGT41100.053.65114237
TCGTATG41800.053.5900240
CATCTCG41500.053.55571736
TATGCCG41700.053.54189743
GCCGTCT39550.053.53256246
CGTCTTC39150.053.45379348
CAGCTTG1900.053.4225554
ATGCCGT41700.053.37405444