FastQCFastQC Report
Wed 25 May 2016
SRR1780875_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780875_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81701
Sequences flagged as poor quality0
Sequence length76
%GC70

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3181438.93954786355124No Hit
CTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTG13731.6805179863159567No Hit
TCTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGT1810.22153951604019537No Hit
CTTATACACATCTGACGCTGCCGCCGATCTACTCTGTGTAGATCTCGGTG1580.19338808582514289No Hit
CTCTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGG1410.17258050697053892No Hit
ATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTGGTC1170.14320510152874505No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCCGG150.002217494270.0000118
TCCCGAT150.002217494270.0000110
TGGCGCT252.3778687E-456.00000413
CGTCTGG252.3778687E-456.0000049
TTTATGT252.3778687E-456.00000448
GATTGAA252.3778687E-456.00000414
CGCGTCT252.3778687E-456.0000047
CCTTACA200.006929060452.549
GCCTTAC200.006929060452.548
TGGACTA200.006929060452.551
TGGCGGC200.006929060452.519
GCGCCCG200.006929060452.539
GTTGTCA200.006929060452.51
CGAGGGG200.006929060452.525
AAAGCTC200.006929060452.556
GTAAACT200.006929060452.533
CTTACAA200.006929060452.550
CCCGATT200.006929060452.511
CAGACCG200.006929060452.513
TGGTCGC2950.051.0169549
Produced by FastQC (version 0.11.5)