##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780875_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 81701 Sequences flagged as poor quality 0 Sequence length 76 %GC 70 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.423764703002412 32.0 27.0 32.0 22.0 32.0 2 29.22511352370228 32.0 32.0 32.0 22.0 32.0 3 28.979045544118186 32.0 32.0 32.0 13.0 32.0 4 28.47086326972742 32.0 27.0 32.0 13.0 32.0 5 28.875607397706272 32.0 32.0 32.0 13.0 32.0 6 32.9426445208749 37.0 32.0 37.0 13.0 37.0 7 32.6216814971665 37.0 32.0 37.0 13.0 37.0 8 32.88853257610066 37.0 32.0 37.0 13.0 37.0 9 33.05465049387401 37.0 32.0 37.0 13.0 37.0 10-11 32.68745180597544 37.0 32.0 37.0 13.0 37.0 12-13 32.52571571951384 37.0 32.0 37.0 13.0 37.0 14-15 33.19817994883783 37.0 34.5 37.0 17.5 37.0 16-17 33.32767040795095 37.0 34.5 37.0 17.5 37.0 18-19 32.82520409786906 37.0 32.0 37.0 13.0 37.0 20-21 33.265057955226986 37.0 34.5 37.0 17.5 37.0 22-23 33.25579246276055 37.0 34.5 37.0 17.5 37.0 24-25 32.59917871262286 37.0 32.0 37.0 13.0 37.0 26-27 33.13657727567594 37.0 34.5 37.0 17.5 37.0 28-29 33.15844359310168 37.0 34.5 37.0 13.0 37.0 30-31 32.90646381317242 37.0 32.0 37.0 13.0 37.0 32-33 33.08442369126449 37.0 34.5 37.0 13.0 37.0 34-35 32.967583016119754 37.0 32.0 37.0 13.0 37.0 36-37 33.48349469406739 37.0 34.5 37.0 22.0 37.0 38-39 33.56138847749722 37.0 34.5 37.0 20.0 37.0 40-41 33.01384315981444 37.0 34.5 37.0 17.5 37.0 42-43 32.89208210425821 37.0 32.0 37.0 13.0 37.0 44-45 32.6812707310804 37.0 32.0 37.0 13.0 37.0 46-47 32.94090035617679 37.0 34.5 37.0 17.5 37.0 48-49 33.46653651730089 37.0 34.5 37.0 17.5 37.0 50-51 33.707451561180406 37.0 37.0 37.0 22.0 37.0 52-53 32.82057135163585 37.0 32.0 37.0 13.0 37.0 54-55 32.97273595182433 37.0 34.5 37.0 13.0 37.0 56-57 32.74084772524204 37.0 34.5 37.0 13.0 37.0 58-59 32.27965997968201 37.0 32.0 37.0 13.0 37.0 60-61 32.104784519161335 37.0 32.0 37.0 13.0 37.0 62-63 31.758803441818337 37.0 32.0 37.0 13.0 37.0 64-65 31.604441806097846 37.0 29.5 37.0 13.0 37.0 66-67 31.547386200903293 37.0 32.0 37.0 13.0 37.0 68-69 31.744654288197204 37.0 32.0 37.0 13.0 37.0 70-71 31.87948739917504 37.0 32.0 37.0 13.0 37.0 72-73 32.29876011309531 37.0 32.0 37.0 13.0 37.0 74-75 32.4972582954921 37.0 32.0 37.0 13.0 37.0 76 32.48726453776575 37.0 32.0 37.0 13.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 11101 1 0.4707496594495062 11101 2 -0.42626341988805905 11101 3 0.23576218901996526 11101 4 1.4923991576906523 11101 5 0.5427721156167529 11101 6 -1.2213705203519751 11101 7 0.1926714214759997 11101 8 0.7492545794168848 11101 9 1.9622502437531253 11101 10-11 0.33403678892013033 11101 12-13 2.024370113557424 11101 14-15 0.6277936887042728 11101 16-17 -0.2811804011584442 11101 18-19 -0.3388964893810851 11101 20-21 -0.2032171451829754 11101 22-23 0.3899995454447094 11101 24-25 1.0548229147187698 11101 26-27 1.5233725674215322 11101 28-29 1.0768662442418346 11101 30-31 1.3837384212999666 11101 32-33 -0.6635195538265535 11101 34-35 0.7900555298356267 11101 36-37 -0.19284395406762656 11101 38-39 -0.5908468499380675 11101 40-41 0.5795596895377457 11101 42-43 0.8345337702817659 11101 44-45 1.63392598553245 11101 46-47 1.4573945479255244 11101 48-49 0.003775681568292555 11101 50-51 0.622627855981456 11101 52-53 2.172006833118921 11101 54-55 -0.744477380542385 11101 56-57 0.5859428851209074 11101 58-59 0.16796177544190982 11101 60-61 0.09230633331039684 11101 62-63 1.8555770893913355 11101 64-65 0.8452448580771694 11101 66-67 0.2974869705207368 11101 68-69 1.3903409815173653 11101 70-71 0.07783617607520554 11101 72-73 0.3848131069593137 11101 74-75 1.8902015346912648 11101 76 -0.3972154851743568 11102 1 0.8000807057616868 11102 2 -0.22214678180915826 11102 3 0.40797488198737497 11102 4 0.6021761731280435 11102 5 0.7654136250507158 11102 6 1.3323173012603746 11102 7 0.7981602722478698 11102 8 2.5238686445969947 11102 9 0.7965555610773123 11102 10-11 0.022887560789769168 11102 12-13 1.1919515886860665 11102 14-15 0.4746204468517874 11102 16-17 0.9272244015859741 11102 18-19 -0.5661692166538117 11102 20-21 0.05767479306745571 11102 22-23 0.21950211834350597 11102 24-25 0.5695742011681659 11102 26-27 0.13040515747299253 11102 28-29 1.7501080967289724 11102 30-31 1.0902564315915697 11102 32-33 -0.24225025708555847 11102 34-35 -0.26569061081617207 11102 36-37 0.10561230665278742 11102 38-39 -0.32412300774252145 11102 40-41 0.27441389193912613 11102 42-43 1.2252713346042299 11102 44-45 0.22020385860277258 11102 46-47 -0.06679070078802596 11102 48-49 -0.34167886388625135 11102 50-51 -0.08629152652282812 11102 52-53 0.5255231281446484 11102 54-55 0.9409428595948341 11102 56-57 0.7224780480711672 11102 58-59 0.11616074628238948 11102 60-61 -0.34182745742716847 11102 62-63 0.45814998819064456 11102 64-65 -0.18065565650259074 11102 66-67 1.6942279653749388 11102 68-69 -0.2117173375220922 11102 70-71 0.4819528141541056 11102 72-73 0.19013043114455996 11102 74-75 1.0841981041595332 11102 76 0.4041567275186111 11103 1 0.0630677187486981 11103 2 -0.0987701584325329 11103 3 0.3823255313380223 11103 4 0.7612670822189536 11103 5 0.9868421964792873 11103 6 0.8800445739876466 11103 7 2.432900531988132 11103 8 -0.10957291384456624 11103 9 1.2806464701682216 11103 10-11 0.08993301533521958 11103 12-13 0.11500353673800845 11103 14-15 -0.111256176524833 11103 16-17 -0.15719118282961375 11103 18-19 1.7414606534760608 11103 20-21 -0.48404598615331906 11103 22-23 -0.3254654141240252 11103 24-25 -0.8788024222084587 11103 26-27 -0.8457312061633715 11103 28-29 0.9570886162094965 11103 30-31 1.2683408471759776 11103 32-33 0.44817182083652085 11103 34-35 0.9088223761968166 11103 36-37 0.8093460728865551 11103 38-39 0.39584452472499976 11103 40-41 0.6344788270040524 11103 42-43 -0.205897496564603 11103 44-45 0.2029960663949808 11103 46-47 0.12265735116002219 11103 48-49 -0.30872431843170745 11103 50-51 -0.30122659145789754 11103 52-53 0.05441923204075039 11103 54-55 0.20279350894548287 11103 56-57 -0.2129115623184461 11103 58-59 -0.3904950978734547 11103 60-61 1.1089842308845164 11103 62-63 -0.5022396221989638 11103 64-65 -0.6616621500090858 11103 66-67 -0.42606424241726515 11103 68-69 -1.0680485063532608 11103 70-71 -0.041099133897841256 11103 72-73 1.1113966649107994 11103 74-75 -0.37310709064566083 11103 76 -3.1630510646891814 11104 1 -1.694868789187808 11104 2 -0.18342624308861843 11104 3 1.17531494932744 11104 4 -1.285029214077344 11104 5 -0.5012530416159535 11104 6 -0.9393998704567963 11104 7 0.4547259288135308 11104 8 -0.37478455405620537 11104 9 1.3315723960941455 11104 10-11 -0.9077521698499638 11104 12-13 0.5773051240395972 11104 14-15 -0.09372972149838432 11104 16-17 0.033285007646583153 11104 18-19 0.448140547655953 11104 20-21 -0.5472073618146993 11104 22-23 -0.3820130331716385 11104 24-25 0.8215944031883708 11104 26-27 -0.1998978728300358 11104 28-29 -0.3163902197693389 11104 30-31 0.06298370431883882 11104 32-33 -1.3100953749306754 11104 34-35 -1.131852226977788 11104 36-37 -0.09640789536742034 11104 38-39 -0.7374226710421894 11104 40-41 -0.23787567035044077 11104 42-43 -0.827590618257716 11104 44-45 -0.6138028754039553 11104 46-47 0.22748539348806673 11104 48-49 -0.3202983925057836 11104 50-51 -0.5948773851086884 11104 52-53 0.5325938352153514 11104 54-55 0.049023667675641036 11104 56-57 0.35917838477150354 11104 58-59 -0.2102702301485877 11104 60-61 -0.5408173564170653 11104 62-63 -0.25885337881272363 11104 64-65 -0.5707229965699305 11104 66-67 -0.6717653006183255 11104 68-69 -0.4625590883638431 11104 70-71 -0.7996970174957241 11104 72-73 -0.6263678853537513 11104 74-75 0.6232553432167691 11104 76 1.3812611046229861 11105 1 1.4747218540870435 11105 2 0.7395757062291217 11105 3 -0.3266343684113515 11105 4 -0.2957504616406972 11105 5 1.1303259057524677 11105 6 0.05767615293501649 11105 7 0.5015095545445192 11105 8 0.9809659332732252 11105 9 1.4144183999927833 11105 10-11 -0.34537400220864356 11105 12-13 0.23373787257760625 11105 14-15 -1.2467511639935083 11105 16-17 0.7438113234360557 11105 18-19 1.161103510618915 11105 20-21 -0.4541274397874133 11105 22-23 0.1486887212143202 11105 24-25 0.5745183797965581 11105 26-27 1.2386986183980326 11105 28-29 0.3761049069363125 11105 30-31 1.2033345815118253 11105 32-33 -0.2365086302912971 11105 34-35 0.02458052156022461 11105 36-37 -0.3712616965370046 11105 38-39 0.809848284123504 11105 40-41 -0.018090095301708686 11105 42-43 0.7113957365181136 11105 44-45 0.58990082829974 11105 46-47 0.28791424339060256 11105 48-49 -0.6453471254492555 11105 50-51 0.17559045114862215 11105 52-53 1.3542312621159454 11105 54-55 -0.1234909522073977 11105 56-57 0.3757475855512311 11105 58-59 1.8837843507481011 11105 60-61 0.1404691932905351 11105 62-63 0.17633180637246326 11105 64-65 -0.5380719244451733 11105 66-67 0.08067134655516384 11105 68-69 -0.11363121312017554 11105 70-71 0.3050788109643179 11105 72-73 -0.5873815305779253 11105 74-75 0.5452826336651171 11105 76 -0.37702349576687766 11106 1 -0.6883328414753898 11106 2 -0.5483499903653062 11106 3 1.3441602652315794 11106 4 0.19754159202287624 11106 5 0.6163940172075755 11106 6 0.0834759465366659 11106 7 -1.1857969470034675 11106 8 -0.9292507849930232 11106 9 -0.8786672553219717 11106 10-11 -0.21276306309615478 11106 12-13 -0.7347863792283746 11106 14-15 -0.6285881092979508 11106 16-17 0.6901477527446147 11106 18-19 -0.24085727369481447 11106 20-21 -1.3767280589824509 11106 22-23 -0.08299342532850318 11106 24-25 0.5095028999203919 11106 26-27 -0.2685253238104295 11106 28-29 -0.5119239888325211 11106 30-31 -0.7605457074458712 11106 32-33 -0.5492021287433104 11106 34-35 -0.38512020083399534 11106 36-37 -0.5931399215112094 11106 38-39 -0.7902549150509017 11106 40-41 0.6243247653794057 11106 42-43 -1.0084185049679562 11106 44-45 0.4918616126134694 11106 46-47 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26-27 1.1213142483820846 11107 28-29 0.641017187638063 11107 30-31 0.6993473406824791 11107 32-33 -0.15134116617646498 11107 34-35 0.8343093891838294 11107 36-37 -0.1852967842563089 11107 38-39 0.5213315377120225 11107 40-41 0.41077752830275216 11107 42-43 -0.6020013926684982 11107 44-45 0.18384022223914087 11107 46-47 1.6423002083028848 11107 48-49 -0.08713340934080094 11107 50-51 0.9773448371135274 11107 52-53 -0.5017495991280754 11107 54-55 0.8318519505039248 11107 56-57 -0.8775219519288342 11107 58-59 1.2797971099187464 11107 60-61 1.6490816334819272 11107 62-63 0.30360453364518847 11107 64-65 0.9466170707701345 11107 66-67 0.6669552381022079 11107 68-69 -0.0662627920675476 11107 70-71 0.254680086881379 11107 72-73 0.12649406750819736 11107 74-75 1.456925376886808 11107 76 1.5314294547913363 11108 1 1.5673534330344125 11108 2 0.06694412728175081 11108 3 -0.10838875437626072 11108 4 0.33126708221895385 11108 5 -0.4945863749492858 11108 6 0.5650445739876488 11108 7 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0.5669441272817508 21612 3 -0.6883887543762626 21612 4 -0.46873291778104687 21612 5 -0.5745863749492841 21612 6 -0.5549554260123557 21612 7 1.8836148177024157 21612 8 -0.5088586281302838 21612 9 1.3656464701682225 21612 10-11 0.22743301533522242 21612 12-13 0.29286067959515094 21612 14-15 0.5455295377608707 21612 16-17 -0.15004832568674686 21612 18-19 0.26110351061891635 21612 20-21 0.21131115670382172 21612 22-23 -0.5886796998383161 21612 24-25 0.030483292077256863 21612 26-27 -0.06323120616337263 21612 28-29 0.2910171876380687 21612 30-31 -0.2970162956811606 21612 32-33 0.551386106550801 21612 34-35 -0.012963338088894716 21612 36-37 0.3047032157437002 21612 38-39 -0.24594118956070332 21612 40-41 -0.41194974442451837 21612 42-43 -0.24018321085031147 21612 44-45 0.4965674949664063 21612 46-47 -0.5876997916971121 21612 48-49 -1.0062243184317055 21612 50-51 -0.8126551628864647 21612 52-53 1.3237049463264654 21612 54-55 0.7745792232311999 21612 56-57 -0.08934013374701522 21612 58-59 -0.6365665264448879 21612 60-61 -0.21637291197262343 21612 62-63 0.7863318063724627 21612 64-65 0.10298070713377427 21612 66-67 0.5878643290113068 21612 68-69 1.0037372079324562 21612 70-71 0.8110437232450209 21612 72-73 -0.7089604779463485 21612 74-75 -0.5848928049313749 21612 76 -0.1394796361177555 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 8.0 12 27.0 13 50.0 14 115.0 15 187.0 16 273.0 17 427.0 18 530.0 19 596.0 20 687.0 21 816.0 22 942.0 23 1204.0 24 1362.0 25 1669.0 26 1867.0 27 2147.0 28 2483.0 29 2777.0 30 3513.0 31 4264.0 32 5316.0 33 7453.0 34 10279.0 35 14144.0 36 18564.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 66.55895420996585 10.747989571475783 5.7993365891871385 16.89371962937123 2 55.41071494143135 12.558293247163368 19.787267898015887 12.243723913389392 3 54.4900357440141 12.89967193850071 19.078979581844 13.531312735641187 4 52.881272949816406 12.849449204406366 17.944920440636476 16.32435740514076 5 51.96817625458996 17.46511627906977 21.72949816401469 8.837209302325581 6 60.42888091945019 21.076853404487093 9.923991138419359 8.570274537643357 7 58.16441441441441 16.933509596553076 10.916079122600863 13.985996866431648 8 57.94492044063647 20.439412484700124 10.8531211750306 10.762545899632803 9 56.6328029375765 10.800489596083231 10.641370869033048 21.925336597307222 10-11 58.38188494492044 15.216034271725826 14.94798041615667 11.454100367197062 12-13 57.30084027442028 13.586374457616019 14.668388423429764 14.444396844533932 14-15 59.29006395544539 13.879249671042565 12.291685792098901 14.53900058141314 16-17 58.66433283963916 14.652924832001174 12.133248466895969 14.549493861463702 18-19 56.6999394113723 14.4476336774849 14.380312980042472 14.472113931100324 20-21 58.64590791978017 14.22023121033531 12.988904596667094 14.14495627321742 22-23 58.694468074689254 14.08287789861503 12.297655403709982 14.924998622985733 24-25 57.475611696593596 15.44143747169488 12.420592663312892 14.662358168398635 26-27 58.652639824725675 15.458289728949026 13.114974816555591 12.774095629769707 28-29 57.4099460226925 14.396396616932472 14.679134894309739 13.514522466065287 30-31 57.28754850001836 15.084270694361146 14.783784776196129 12.844396029424365 32-33 57.0559543699243 14.450339961199749 14.731855986193477 13.761849682682481 34-35 57.001481082536685 13.867706280524377 14.88855159920193 14.242261037737002 36-37 58.36464899932676 13.645877960707509 15.47707938062305 12.512393659342678 38-39 58.80758144174689 13.485394647458063 15.777330338618963 11.929693572176085 40-41 58.34093259809172 14.62914567943547 14.755222071936986 12.274699650535826 42-43 56.778986793307304 14.296030648339677 16.143009265492466 12.781973292860553 44-45 55.96621683650051 13.173597723308546 16.64065607882738 14.219529361363566 46-47 56.968083478686616 13.137488907249303 15.470485632975306 14.423941981088772 48-49 57.192254495159055 12.732101984112413 17.204004944980966 12.87163857574756 50-51 58.19716275597009 13.02892324263455 15.512429772702236 13.26148422869313 52-53 55.011138583563856 14.033391270288135 16.604445641264167 14.351024504883842 54-55 56.01101591187271 14.08873929008568 15.12607099143207 14.774173806609546 56-57 56.2301864159904 14.23946437532895 14.503849496321862 15.02649971235878 58-59 55.247952600717355 15.366205976324842 15.936662218903402 13.449179204054401 60-61 54.88460902932051 14.194527573270665 16.050085372794538 14.870778024614292 62-63 55.340946645613776 15.079131935519408 15.304348890439295 14.27557252842752 64-65 55.52318642781514 15.104686247268855 15.599811497432569 13.77231582748343 66-67 55.510741171430325 16.791725319787012 13.238876308219597 14.458657200563069 68-69 55.94567798477319 16.61546182281084 13.089059707703981 14.349800484711988 70-71 55.88694326966037 16.674521228739128 13.051221945442414 14.38731355615808 72-73 56.51748174713431 16.07598577714674 13.032515498870861 14.374016976848084 74-75 57.038206620522516 15.202477341019947 13.275316552530278 14.48399948592726 76 58.9454229446396 13.009632685034456 13.396408856684742 14.648535513641203 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 0.5 7 0.5 8 1.0 9 0.5 10 1.0 11 2.5 12 3.0 13 4.0 14 5.0 15 13.5 16 29.0 17 36.0 18 41.0 19 56.5 20 69.0 21 71.0 22 102.5 23 165.0 24 236.5 25 277.0 26 262.5 27 288.5 28 432.0 29 535.0 30 574.0 31 633.5 32 726.0 33 798.0 34 873.5 35 1039.5 36 1157.0 37 1184.0 38 1288.5 39 1482.0 40 1621.5 41 1938.5 42 2205.0 43 2647.0 44 2880.5 45 2477.0 46 2282.0 47 2210.0 48 2053.5 49 1932.5 50 1896.0 51 1923.5 52 1930.5 53 1887.0 54 1864.0 55 1816.5 56 1680.5 57 1496.0 58 1400.0 59 1211.5 60 949.5 61 788.5 62 701.0 63 648.5 64 570.5 65 498.0 66 383.5 67 316.0 68 285.0 69 237.0 70 226.0 71 232.0 72 214.0 73 171.0 74 136.0 75 126.0 76 120.5 77 111.0 78 100.0 79 93.0 80 90.5 81 94.0 82 97.5 83 95.0 84 95.0 85 103.5 86 122.0 87 132.0 88 130.0 89 151.0 90 177.5 91 192.5 92 204.0 93 228.5 94 307.5 95 406.5 96 451.0 97 597.0 98 1135.0 99 16953.5 100 32380.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0024479504534828216 2 0.0024479504534828216 3 0.011015777040672695 4 0.0012239752267414108 5 0.0012239752267414108 6 0.0 7 0.006119876133707054 8 0.0012239752267414108 9 0.0012239752267414108 10-11 0.0012239752267414108 12-13 0.0018359628401121162 14-15 0.004283913293594938 16-17 0.004895900906965643 18-19 0.003059938066853527 20-21 6.119876133707054E-4 22-23 0.003059938066853527 24-25 0.0024479504534828216 26-27 6.119876133707054E-4 28-29 0.0 30-31 0.0 32-33 6.119876133707054E-4 34-35 0.004895900906965643 36-37 0.007343851360448465 38-39 0.0018359628401121162 40-41 0.005507888520336348 42-43 0.0 44-45 0.004283913293594938 46-47 0.004283913293594938 48-49 0.0024479504534828216 50-51 0.0024479504534828216 52-53 0.0036719256802242323 54-55 0.0012239752267414108 56-57 0.0024479504534828216 58-59 0.01468770272089693 60-61 6.119876133707054E-4 62-63 0.0024479504534828216 64-65 0.005507888520336348 66-67 0.007343851360448465 68-69 0.0036719256802242323 70-71 0.00917981420056058 72-73 0.0018359628401121162 74-75 6.119876133707054E-4 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 81701.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.25781814176081 #Duplication Level Percentage of deduplicated Percentage of total 1 76.4878641596057 27.732830687506887 2 8.614927590048273 6.24716955728816 3 5.7185295209803195 6.220242102299849 4 3.8787428687168752 5.625390142103524 5 2.4035377915808662 4.357351807199422 6 1.3334233534753401 2.9008212873771435 7 0.6785268203760592 1.722133144025165 8 0.37133308577794283 1.0770981995324413 9 0.12827870235965297 0.4185995275455625 >10 0.35107855382641867 2.0256790002570346 >50 0.013503021301016103 0.321905484632991 >100 0.013503021301016103 0.7307132103646222 >500 0.0 0.0 >1k 0.003375755325254026 1.6805179863159567 >5k 0.0 0.0 >10k+ 0.003375755325254026 38.93954786355124 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 31814 38.93954786355124 No Hit CTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTG 1373 1.6805179863159567 No Hit TCTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGT 181 0.22153951604019537 No Hit CTTATACACATCTGACGCTGCCGCCGATCTACTCTGTGTAGATCTCGGTG 158 0.19338808582514289 No Hit CTCTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGG 141 0.17258050697053892 No Hit ATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTGGTC 117 0.14320510152874505 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.02080757885460398 0.0 2 0.0 0.0 0.0 0.02080757885460398 0.0 3 0.0 0.0 0.0 0.02080757885460398 0.0 4 0.0 0.0 0.0 0.0342713063487595 0.0 5 0.0 0.0 0.0 0.046511058616173606 0.0 6 0.0 0.0 0.0 0.046511058616173606 0.0 7 0.0 0.0 0.0 0.046511058616173606 0.0 8 0.0 0.0 0.0 0.046511058616173606 0.0 9 0.0 0.0 0.0 0.04773503384291502 0.0 10 0.0 0.0 0.0 0.050182984296397844 0.0 11 0.0 0.0 0.0 0.05140695952313925 0.0 12 0.0 0.0 0.0 0.061198761337070536 0.0 13 0.0 0.0 0.0 0.06487068701729477 0.0 14 0.0 0.0 0.0 0.06976658792426041 0.0 15 0.0 0.0 0.0 0.07343851360448464 0.0 16 0.0 0.0 0.0 0.07343851360448464 0.0 17 0.0 0.0 0.0 0.07343851360448464 0.0 18 0.0 0.0 0.0 0.07343851360448464 0.0 19 0.0 0.0 0.0 0.08690224109864016 0.0 20 0.0 0.0 0.0 0.08812621632538158 0.0 21 0.0 0.0 0.0 0.09302211723234721 0.0 22 0.0 0.0 0.0 0.11750162176717543 0.0 23 0.0 0.0 0.0 0.12117354744739967 0.0 24 0.0 0.0 0.0 0.13341329971481378 0.0 25 0.0 0.0 0.0 0.13830920062177943 0.0 26 0.0 0.0 0.0 0.13830920062177943 0.0 27 0.0 0.0 0.0 0.1811483335577288 0.0 28 0.0 0.0 0.0 0.19583603627862572 0.0 29 0.0 0.0 0.0 0.24479504534828214 0.0 30 0.0 0.0 0.0 0.3439370387143364 0.0 31 0.0 0.0 0.0 0.38677617165028577 0.0 32 0.0 0.0 0.0 0.4675585366152189 0.0 33 0.0 0.0 0.0 0.5434450006731864 0.0 34 0.0 0.0 0.0 0.7099056315100183 0.0 35 0.0 0.0 0.0 0.8629025348526946 0.0 36 0.0 0.0 0.0 1.0660824224917689 0.0 37 0.0 0.0 0.0 1.611975373618438 0.0 38 0.0 0.0 0.0 2.2117232347217293 0.0 39 0.0 0.0 0.0 2.851862278307487 0.0 40 0.0 0.0 0.0 3.4479382137305543 0.0 41 0.0 0.0 0.0 4.193339126816073 0.0 42 0.0 0.0 0.0 4.964443519663162 0.0 43 0.0 0.0 0.0 5.6902608291208185 0.0 44 0.0 0.0 0.0 6.545819512613065 0.0 45 0.0 0.0 0.0 7.413617948372725 0.0 46 0.0 0.0 0.0 8.174930539405882 0.0 47 0.0 0.0 0.0 8.898299898410055 0.0 48 0.0 0.0 0.0 9.53599099154233 0.0 49 0.0 0.0 0.0 10.222641093744263 0.0 50 0.0 0.0 0.0 10.80158137599295 0.0 51 0.0 0.0 0.0 11.42580874163107 0.0 52 0.0 0.0 0.0 12.014540825693688 0.0 53 0.0 0.0 0.0 12.5971530336226 0.0 54 0.0 0.0 0.0 13.043903991383214 0.0 55 0.0 0.0 0.0 13.505342651864726 0.0 56 0.0 0.0 0.0 14.041443801177465 0.0 57 0.0 0.0 0.0 14.5065543873392 0.0 58 0.0 0.0 0.0 14.952081369873074 0.0 59 0.0 0.0 0.0 15.355993194697739 0.0 60 0.0 0.0 0.0 15.762352969975888 0.0 61 0.0 0.0 0.0 16.150353116852916 0.0 62 0.0 0.0 0.0 16.513873759195114 0.0 63 0.0 0.0 0.0 16.92023353447326 0.0 64 0.0 0.0 0.0 17.253154796146926 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGCCGG 15 0.0022174942 70.00001 18 TCCCGAT 15 0.0022174942 70.00001 10 TGGCGCT 25 2.3778687E-4 56.000004 13 CGTCTGG 25 2.3778687E-4 56.000004 9 TTTATGT 25 2.3778687E-4 56.000004 48 GATTGAA 25 2.3778687E-4 56.000004 14 CGCGTCT 25 2.3778687E-4 56.000004 7 CCTTACA 20 0.0069290604 52.5 49 GCCTTAC 20 0.0069290604 52.5 48 TGGACTA 20 0.0069290604 52.5 51 TGGCGGC 20 0.0069290604 52.5 19 GCGCCCG 20 0.0069290604 52.5 39 GTTGTCA 20 0.0069290604 52.5 1 CGAGGGG 20 0.0069290604 52.5 25 AAAGCTC 20 0.0069290604 52.5 56 GTAAACT 20 0.0069290604 52.5 33 CTTACAA 20 0.0069290604 52.5 50 CCCGATT 20 0.0069290604 52.5 11 CAGACCG 20 0.0069290604 52.5 13 TGGTCGC 295 0.0 51.01695 49 >>END_MODULE