FastQCFastQC Report
Wed 25 May 2016
SRR1780875_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780875_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81701
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCCGT2139726.18939792658597No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCCG30633.749036119508941No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26163.201919193155531No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCC20562.5164930661803404No Hit
ATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCCGTCTT5960.7294892351378808RNA PCR Primer, Index 45 (95% over 22bp)
CATATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCCGT4280.5238613970453239No Hit
CTTATACACATCTCCGCGCCCACGAGACGCCAATTCATCTCGTATGCCGT3880.47490238797566736No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCC3290.40268784959792414No Hit
CTAATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCCGT2490.3047698314586113No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGACGT2140.26193069852266193No Hit
CTTATACACATCTCCGAGCCCACGAGCCGCCAATTCATCTCGTATGCCGT1700.2080757885460398No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCAGT1530.18726820969143584No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCAATTCATATCGTATGCCGT1460.178700383104246No Hit
CTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCCGTC1390.1701325565170561No Hit
ATTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCCGT1370.16768460606357327No Hit
CTTTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCCG1070.13096534926133097No Hit
CCTTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCCG1050.12851739880784813No Hit
CTTTACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCCGTC1030.12606944835436532No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCCGA930.1138296960869512No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCAATTCCTCTCGTATGCCGT900.11015777040672696No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCAAATCATCTCGTATGCCGT850.1040378942730199Illumina PCR Primer Index 7 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGTC150.00221757169.9993945
ATCTCGA252.8175727E-669.9993937
CATGCCA150.00221757169.9993926
ACCTGCA150.00221757169.999397
TTGTAAG150.00221757169.9993935
TATGAGT150.00221757169.9993937
AAGCCGT150.00221757169.9993944
CTCGAAT252.8175727E-669.9993939
GACGCAA207.885027E-569.9993926
TGTACTT150.00221757169.999395
GTGACAA150.00221757169.9993954
AGGGGGG26050.063.95337370
CGTCTTA501.4551915E-1162.9994548
CCGACTT401.0508302E-861.24946647
CGACTTC401.0508302E-861.24946648
CAAATAC352.929355E-760.0362171
CCGTCTA352.941797E-759.99947747
TCTTATG601.8189894E-1258.33282550
AATGCCG308.311345E-658.33282543
ACTCGTA308.311345E-658.33282538
Produced by FastQC (version 0.11.5)