##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780875_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 81701 Sequences flagged as poor quality 0 Sequence length 76 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.976303839610285 32.0 27.0 32.0 13.0 32.0 2 29.716686454266167 32.0 32.0 32.0 27.0 32.0 3 30.099435747420472 32.0 32.0 32.0 27.0 32.0 4 30.56993182457987 32.0 32.0 32.0 27.0 32.0 5 30.445624900552012 32.0 32.0 32.0 27.0 32.0 6 34.06571522992375 37.0 37.0 37.0 27.0 37.0 7 33.99806611914175 37.0 37.0 37.0 27.0 37.0 8 33.534522221270244 37.0 32.0 37.0 22.0 37.0 9 34.054111944774235 37.0 37.0 37.0 27.0 37.0 10-11 34.08704911812585 37.0 34.5 37.0 24.5 37.0 12-13 34.46418648486555 37.0 37.0 37.0 27.0 37.0 14-15 34.22360191429725 37.0 37.0 37.0 27.0 37.0 16-17 32.73060917247035 37.0 29.5 37.0 20.0 37.0 18-19 33.77689379566958 37.0 34.5 37.0 27.0 37.0 20-21 34.204715976548634 37.0 37.0 37.0 27.0 37.0 22-23 33.410521291049065 37.0 34.5 37.0 24.5 37.0 24-25 33.42183694202029 37.0 32.0 37.0 24.5 37.0 26-27 33.65527349726442 37.0 34.5 37.0 20.0 37.0 28-29 34.234213779513105 37.0 37.0 37.0 27.0 37.0 30-31 34.340632305602135 37.0 37.0 37.0 27.0 37.0 32-33 33.56301024467265 37.0 32.0 37.0 22.0 37.0 34-35 33.969418978959865 37.0 37.0 37.0 24.5 37.0 36-37 32.675640445037395 37.0 32.0 37.0 20.0 37.0 38-39 31.472466677274454 37.0 29.5 37.0 13.0 37.0 40-41 32.55122336323913 37.0 32.0 37.0 17.5 37.0 42-43 33.847003096657325 37.0 37.0 37.0 27.0 37.0 44-45 33.47711166325993 37.0 34.5 37.0 22.0 37.0 46-47 33.35087697824996 37.0 34.5 37.0 24.5 37.0 48-49 32.673902400215425 37.0 32.0 37.0 13.0 37.0 50-51 33.05407522551744 37.0 32.0 37.0 22.0 37.0 52-53 32.44131038787775 37.0 32.0 37.0 13.0 37.0 54-55 32.27630628756074 37.0 32.0 37.0 13.0 37.0 56-57 32.57768570764128 37.0 32.0 37.0 13.0 37.0 58-59 32.27926218773332 37.0 32.0 37.0 13.0 37.0 60-61 32.878508218993645 37.0 32.0 37.0 17.5 37.0 62-63 33.05483409015801 37.0 32.0 37.0 22.0 37.0 64-65 33.04081345393569 37.0 32.0 37.0 22.0 37.0 66-67 32.81188112752598 37.0 32.0 37.0 13.0 37.0 68-69 31.908434413287477 37.0 32.0 37.0 13.0 37.0 70-71 29.329212616736637 34.5 24.5 37.0 13.0 37.0 72-73 31.382963488818987 37.0 29.5 37.0 13.0 37.0 74-75 32.67056094784641 37.0 32.0 37.0 13.0 37.0 76 32.973035825754884 37.0 32.0 37.0 13.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 11101 1 0.9066328454140233 11101 2 -1.8320401066996368 11101 3 0.04223546910372278 11101 4 -0.6374909577419956 11101 5 0.1993392525069524 11101 6 -0.25075549736612857 11101 7 0.9966301075288868 11101 8 -1.263005735230628 11101 9 0.17008099852701974 11101 10-11 -0.14283832287551945 11101 12-13 -0.46120877236975133 11101 14-15 -1.5833071013143183 11101 16-17 -0.08915738380709115 11101 18-19 -0.11966620220979962 11101 20-21 0.4118947558653616 11101 22-23 -1.2282186273581601 11101 24-25 -0.3464298539473063 11101 26-27 -0.06860036083126175 11101 28-29 -0.6833369695349631 11101 30-31 -0.4806803938254589 11101 32-33 0.29682488769320514 11101 34-35 -1.078572557049661 11101 36-37 -1.012898728539227 11101 38-39 -0.26012403005771745 11101 40-41 0.3895615782822688 11101 42-43 -0.21897162703708517 11101 44-45 0.6326812182829258 11101 46-47 -0.2696729430658422 11101 48-49 -0.18726301048411642 11101 50-51 0.10255988753119283 11101 52-53 -0.6645785590021589 11101 54-55 -1.9152621983006064 11101 56-57 -0.03050294291610811 11101 58-59 0.8589487637518118 11101 60-61 -0.9674733214728448 11101 62-63 -0.1909036474314192 11101 64-65 -0.1450068351538505 11101 66-67 -2.4761543796703904 11101 68-69 0.17410325295421814 11101 70-71 -2.9763760187697272 11101 72-73 0.16766852300124313 11101 74-75 -0.5543785111801327 11101 76 -0.4170371635115657 11102 1 0.33098962071419535 11102 2 0.06573004767429325 11102 3 -0.562567275321662 11102 4 -0.32771394230460515 11102 5 -0.8589797869441611 11102 6 -0.9179939193215318 11102 7 -0.2565431343236 11102 8 -0.4859902978378372 11102 9 -0.25907852119853914 11102 10-11 -1.4545021307657393 11102 12-13 0.1027706444398575 11102 14-15 -0.2675266553451934 11102 16-17 -0.27337693783796624 11102 18-19 -0.5261842125014056 11102 20-21 0.6671263167572974 11102 22-23 -0.21243818138903947 11102 24-25 -0.8107523239301528 11102 26-27 -2.823488868549962 11102 28-29 -0.3109527499809346 11102 30-31 -0.5206460885081299 11102 32-33 0.3680084211408854 11102 34-35 0.21748233145806495 11102 36-37 -0.20380781944831483 11102 38-39 0.5367884913831062 11102 40-41 -0.990198284496465 11102 42-43 -0.5644261724916291 11102 44-45 -0.9966498280292484 11102 46-47 0.38795999003879444 11102 48-49 0.5353784989498465 11102 50-51 -0.246668242829017 11102 52-53 -0.42324065162652147 11102 54-55 -0.5322433303760761 11102 56-57 -0.421755086998445 11102 58-59 -0.8784440321315543 11102 60-61 -1.2697031670989176 11102 62-63 -0.18369953079334067 11102 64-65 0.896845651981657 11102 66-67 -1.5874751343873683 11102 68-69 -0.689190057508906 11102 70-71 -2.89044119887264 11102 72-73 -1.4141496588169389 11102 74-75 -0.20292053519385433 11102 76 0.016238994292891107 11103 1 0.7728727375973143 11103 2 -0.6076465757023328 11103 3 0.249445711691326 11103 4 -0.6364801760708367 11103 5 0.0419942390298651 11103 6 0.16804504171743417 11103 7 -1.2156340434145108 11103 8 0.9084902216426869 11103 9 -0.16752007964009863 11103 10-11 -0.5082358969995084 11103 12-13 0.29822518989440283 11103 14-15 -0.0868448371633761 11103 16-17 -1.4230522625132949 11103 18-19 0.33501708619989756 11103 20-21 -0.26566589103490656 11103 22-23 0.6432436367927821 11103 24-25 -1.5081549213327463 11103 26-27 0.4487513911902994 11103 28-29 0.7000862110580215 11103 30-31 -0.5261655690276115 11103 32-33 -0.8081279424954744 11103 34-35 -0.39242026594452994 11103 36-37 -0.6736130142535117 11103 38-39 -1.5503868332922188 11103 40-41 -1.8663346481328276 11103 42-43 -0.5493287698942311 11103 44-45 -0.33463684101626257 11103 46-47 0.002732717311516808 11103 48-49 -0.09140721533586316 11103 50-51 -0.6440708402316062 11103 52-53 -0.44742896331483095 11103 54-55 -0.5072433303760775 11103 56-57 0.7100630948197377 11103 58-59 -2.463995980183501 11103 60-61 -0.8323655047612526 11103 62-63 -0.39457615416996816 11103 64-65 0.36307941821542045 11103 66-67 -1.5285465629588018 11103 68-69 -0.5128913562102007 11103 70-71 -1.2024541858856281 11103 72-73 -0.27876004842732627 11103 74-75 0.39643011415679297 11103 76 0.8730571761110753 11104 1 0.7616293513539283 11104 2 0.115898397842642 11104 3 -1.219469632224019 11104 4 -1.396400810991473 11104 5 1.3177878898235136 11104 6 0.6964841951565859 11104 7 0.6825141047336345 11104 8 -0.06174787359540801 11104 9 -0.13180579392581393 11104 10-11 -1.028407854671464 11104 12-13 0.7611881528573647 11104 14-15 0.026581088762547722 11104 16-17 -0.5903803048413323 11104 18-19 -0.46557815189534324 11104 20-21 0.5140960137269914 11104 22-23 -0.6275896965405465 11104 24-25 -0.1523348155126456 11104 26-27 -0.20766395272504923 11104 28-29 -0.0075857466139339635 11104 30-31 -1.1908481087101563 11104 32-33 -0.9308131276806577 11104 34-35 -0.9926186786429483 11104 36-37 -0.14252835816885323 11104 38-39 -0.32651117191655743 11104 40-41 -0.22050131479949897 11104 42-43 -0.38563829370374947 11104 44-45 -1.1368855182649398 11104 46-47 -0.11069320861440701 11104 48-49 0.4538970174683641 11104 50-51 -0.8993618455226198 11104 52-53 0.7685101901243172 11104 54-55 0.0714603733276249 11104 56-57 0.5156186503752949 11104 58-59 -0.4749086785962007 11104 60-61 -0.3806459280416732 11104 62-63 -1.1409385880324017 11104 64-65 -0.23480417966817413 11104 66-67 -0.8985862454984854 11104 68-69 -0.16831463663348245 11104 70-71 -1.465020323451764 11104 72-73 0.880631485964205 11104 74-75 -0.4170619493352703 11104 76 -1.5019428238889247 11105 1 -0.16916986891897778 11105 2 0.49699001577636537 11105 3 0.1908617517915765 11105 4 0.03049977380385016 11105 5 0.4025832114609429 11105 6 0.14580193394800744 11105 7 -0.29896737674784646 11105 8 -0.5529759437708464 11105 9 -1.728297021995992 11105 10-11 0.5890385390907227 11105 12-13 0.01313747059615622 11105 14-15 -1.0440816792686363 11105 16-17 -0.3130898564982587 11105 18-19 0.49933412880641725 11105 20-21 0.553569697937526 11105 22-23 1.284691005213837 11105 24-25 -0.5670521644405184 11105 26-27 -1.3938237967796283 11105 28-29 -0.1401393528517474 11105 30-31 0.35447352871675264 11105 32-33 -0.15352267933758412 11105 34-35 0.9138140698950608 11105 36-37 0.5485047802327045 11105 38-39 -0.9152051290315697 11105 40-41 1.3409021939724326 11105 42-43 -0.28037513580901674 11105 44-45 1.8480072692594263 11105 46-47 0.20119762959221532 11105 48-49 0.4090627094761601 11105 50-51 0.8233477061343066 11105 52-53 0.0906349464596019 11105 54-55 -0.4392608742357247 11105 56-57 0.9074315158723678 11105 58-59 0.18152406994181547 11105 60-61 0.11594276591543462 11105 62-63 -1.1594571065509207 11105 64-65 0.5761119996690596 11105 66-67 -1.2085277659663198 11105 68-69 0.9905547590780195 11105 70-71 -2.22281759440693 11105 72-73 -0.20075252963033563 11105 74-75 0.3519438986179466 11105 76 -0.10720598178366458 11106 1 -0.884340147556749 11106 2 -0.584537332004853 11106 3 -0.5016047084767408 11106 4 -1.187250484194088 11106 5 -2.6593362931830207 11106 6 -0.8253022972181441 11106 7 -0.632300710081175 11106 8 -1.1009635598699177 11106 9 -1.7592567743179686 11106 10-11 -0.19888715750370523 11106 12-13 -1.5792257904977518 11106 14-15 -0.7473840528496467 11106 16-17 -0.3610774725973229 11106 18-19 -1.2498918773855365 11106 20-21 -0.03982555490045314 11106 22-23 -1.353079892618986 11106 24-25 -0.3162781706324722 11106 26-27 -0.4113676564287445 11106 28-29 -1.261398382779511 11106 30-31 -0.5454232721088488 11106 32-33 -1.887784805240571 11106 34-35 -0.36843567210700456 11106 36-37 -0.8070163755980531 11106 38-39 -0.52150027867037 11106 40-41 -1.1812856285249822 11106 42-43 -0.8660304505664982 11106 44-45 -2.18862843765492 11106 46-47 -0.5867280670022055 11106 48-49 -1.5057979716383905 11106 50-51 -0.4718019326685834 11106 52-53 0.14378252127900737 11106 54-55 -0.4949884284152972 11106 56-57 -0.49091729733712697 11106 58-59 -0.6105295936288755 11106 60-61 -2.0631078016800224 11106 62-63 -1.0025943614528785 11106 64-65 -0.44504383108429835 11106 66-67 0.01644643424008052 11106 68-69 -1.0713647455659476 11106 70-71 0.18585113624322247 11106 72-73 -1.04801775150856 11106 74-75 -1.923597897047685 11106 76 -1.4137075297712762 11107 1 -1.5962831065585306 11107 2 -2.752451770507527 11107 3 -0.308021820776208 11107 4 -0.10953212412278646 11107 5 0.3410202130558382 11107 6 -1.8998121011397089 11107 7 -2.420179497959964 11107 8 -0.7405357523832876 11107 9 -0.16816943028945275 11107 10-11 -0.8817748580384688 11107 12-13 -0.8790475373783266 11107 14-15 0.42338243556390154 11107 16-17 -0.8642860287470597 11107 18-19 -0.4989114852286747 11107 20-21 -0.751055501424517 11107 22-23 0.24210727315641378 11107 24-25 -0.7652977784756061 11107 26-27 -0.8871252321863281 11107 28-29 0.1799563409281575 11107 30-31 -0.12973699759903923 11107 32-33 -0.30471885158638656 11107 34-35 -0.4916085776328458 11107 36-37 0.2689194532789543 11107 38-39 -0.008666054071440499 11107 40-41 -0.7356528299510074 11107 42-43 0.6173920093265508 11107 44-45 0.23971380833438616 11107 46-47 0.5697781718569743 11107 48-49 -0.5282578646865161 11107 50-51 -0.8739409701017422 11107 52-53 -1.150513378899248 11107 54-55 -0.3504251485578962 11107 56-57 -0.11266417790753991 11107 58-59 -0.5602622139497342 11107 60-61 0.9848422874465399 11107 62-63 -0.16551771261152481 11107 64-65 0.28775474289074765 11107 66-67 -0.305656952569187 11107 68-69 0.6199008515820061 11107 70-71 1.3550133465819059 11107 72-73 0.704032159364882 11107 74-75 0.89707946480614 11107 76 0.7253299033838019 11108 1 0.11644416616874054 11108 2 -0.10336086141661838 11108 3 -1.769840002594389 11108 4 0.0813769667863049 11108 5 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11109 44-45 0.7741906522451885 11109 46-47 -1.5526918109903711 11109 48-49 -0.8382064067105333 11109 50-51 -1.5672514332235252 11109 52-53 -1.1551445967380118 11109 54-55 -1.0756395567911738 11109 56-57 -0.936163320274602 11109 58-59 -1.11274934945574 11109 60-61 -1.1184167176992617 11109 62-63 -1.4550545908276504 11109 64-65 -1.43745966534253 11109 66-67 0.25969467693338544 11109 68-69 -0.4391042942155927 11109 70-71 -1.5424137546187815 11109 72-73 -1.671954118508193 11109 74-75 -0.6487181338216423 11109 76 -0.32269754087005964 11110 1 -0.5835558338312588 11110 2 0.7966391385833838 11110 3 -0.9898400025943879 11110 4 0.6013769667863045 11110 5 -0.5444343323987084 11110 6 0.6601878988602863 11110 7 -1.745634043414512 11110 8 -0.7950812069287423 11110 9 0.42819420607418834 11110 10-11 0.596406960143355 11110 12-13 0.6182251898944031 11110 14-15 -0.889344837163371 11110 16-17 -1.2351951196561473 11110 18-19 -0.7589114852286727 11110 20-21 1.1107626803936625 11110 22-23 0.6557436367927849 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-0.12984000259438844 21612 4 0.4013769667863052 21612 5 0.1355656676012913 21612 6 -0.11981210113971485 21612 7 -1.9456340434145076 21612 8 -0.8550812069287446 21612 9 -0.5518057939258156 21612 10-11 0.4764069601433505 21612 12-13 -0.5117748101055994 21612 14-15 0.640655162836623 21612 16-17 0.014804880343852744 21612 18-19 -0.3389114852286781 21612 20-21 -0.3492373196063383 21612 22-23 -0.39425636320721935 21612 24-25 0.6047022215243913 21612 26-27 -0.42803432309541023 21612 28-29 0.07722906820087871 21612 30-31 -0.9297369975990435 21612 32-33 0.4043720575045242 21612 34-35 0.7629368769126117 21612 36-37 0.9371012714607758 21612 38-39 1.6349703095649275 21612 40-41 -1.1138346481328263 21612 42-43 0.30102837296291085 21612 44-45 -1.4239225553019779 21612 46-47 -0.004767282688483476 21612 48-49 -1.4546215010501555 21612 50-51 -0.0712136973744677 21612 52-53 -0.16778610617197387 21612 54-55 -0.46224333037607934 21612 56-57 0.5000630948197369 21612 58-59 1.1606468769593548 21612 60-61 0.9912059238101705 21612 62-63 0.3972095601157477 21612 64-65 0.42593656107256805 21612 66-67 1.262524865612626 21612 68-69 0.17353721521836718 21612 70-71 0.8168315284000869 21612 72-73 -1.1723314769987567 21612 74-75 0.33071582844250713 21612 76 -1.041942823888924 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 5.0 17 10.0 18 18.0 19 38.0 20 60.0 21 124.0 22 224.0 23 360.0 24 538.0 25 824.0 26 1251.0 27 1748.0 28 2399.0 29 3321.0 30 4703.0 31 6815.0 32 10096.0 33 15033.0 34 19066.0 35 13202.0 36 1866.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 22.91483005191498 15.117788226075032 11.178861788617885 50.78851993339211 2 14.698718497937602 14.198112630200367 52.048322541951755 19.054846329910283 3 14.173633125665535 15.566516933697262 52.62236692329347 17.63748301734373 4 11.72078677127575 43.87951187867958 26.93112691399126 17.468574436053412 5 11.370729856427705 24.24082936561364 54.1364242787726 10.252016499186055 6 18.64060319714069 56.183749908198486 15.453254669636957 9.722392225023867 7 17.55777516647082 22.81996670583627 15.601743047395223 44.02051508029769 8 16.363324806305922 55.58561094723443 11.840736343496408 16.210327902963247 9 15.854539223246306 15.555881956939496 10.964638490067198 57.624940329747 10-11 16.71032178308711 34.699085690505626 30.841727763430065 17.7488647629772 12-13 17.45005875440658 14.251003721112419 34.92765863689777 33.37127888758323 14-15 16.533457362822976 13.089191074772646 16.748265015116093 53.62908654728828 16-17 35.81504942011689 31.03828146516111 12.492426328835032 20.65424278588696 18-19 37.795743014161395 18.685817799047747 13.310118603199472 30.208320583591387 20-21 18.528220130482147 14.258785512319912 12.565944893937353 54.647049463260586 22-23 16.644328982074775 33.95511655375426 12.991964553460505 36.40858991071046 24-25 36.09791921664627 33.272337821297434 12.851285189718482 17.778457772337823 26-27 38.180627551301434 34.987178467168924 13.134880077357602 13.697313904172049 28-29 35.33379030086416 18.374378809762785 13.17045704913217 33.121373840240885 30-31 20.531823745410037 14.533047735618116 13.682374541003671 51.25275397796818 32-33 15.530979950549584 50.803569242821126 13.854684325197681 19.810766481431614 34-35 15.873734715609356 20.968531596920478 50.731937185591356 12.425796501878802 36-37 15.6179238932204 30.766453286985474 20.989339175775083 32.62628364401905 38-39 15.766926358178445 18.192889797367183 33.77193250876688 32.26825133568749 40-41 15.6300875206561 13.527755676601997 36.27027357855438 34.57188322418753 42-43 35.21851686383471 12.764616241424262 33.70747320942245 18.309393685318582 44-45 16.96185953144508 32.04607211926852 36.78058214399373 14.211486205292664 46-47 34.62793074536252 14.671015991725675 20.778839268774824 29.922213994136982 48-49 32.946965153425296 12.733626271404267 16.436151332297033 37.883257242873405 50-51 17.99388004895961 13.24908200734394 35.593023255813954 33.1640146878825 52-53 13.282211029443264 14.167773364586076 54.29102993286372 18.258985673106935 54-55 12.899336581066855 14.081740067076307 39.29777962740826 33.721143724448574 56-57 32.407537746877125 13.519104479438893 20.485216443990183 33.588141329693805 58-59 15.089720678596782 14.916521824279663 50.89598276579598 19.097774731327572 60-61 33.04549515917798 31.948201368404305 20.63928226092704 14.36702121149068 62-63 15.139961567177881 55.99441867296606 13.366421463629576 15.499198296226485 64-65 13.238183253667582 58.43283901806097 12.499464474787473 15.829513253483974 66-67 13.552185461266356 58.57293234923316 11.927930574425636 15.946951615074848 68-69 14.89142941075669 57.82822100908223 11.669808318441088 15.610541261719993 70-71 30.4865658853051 42.21433380255829 11.187343166656467 16.11175714548014 72-73 57.415453911212836 15.360277107991335 10.871347963917211 16.352921016878618 74-75 59.616385034854616 13.544643895393316 10.896427631538682 15.942543438213388 76 59.86044803525523 13.411678295997062 11.040519035377647 15.687354633370058 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 1.0 12 1.0 13 3.0 14 8.5 15 12.0 16 14.0 17 16.0 18 25.0 19 46.0 20 67.0 21 76.0 22 86.5 23 122.0 24 171.0 25 195.0 26 201.0 27 252.5 28 334.0 29 370.0 30 440.0 31 543.5 32 661.0 33 745.0 34 735.5 35 875.0 36 1080.5 37 1137.0 38 1313.5 39 1486.0 40 1594.5 41 1784.5 42 1862.0 43 2087.5 44 2464.5 45 3295.5 46 3975.0 47 7567.5 48 17286.0 49 13538.5 50 3665.0 51 3198.0 52 2406.0 53 1989.5 54 1898.0 55 1897.5 56 1820.5 57 1626.0 58 1508.0 59 1448.5 60 1256.0 61 1022.0 62 921.0 63 814.0 64 644.0 65 526.0 66 438.5 67 406.0 68 355.0 69 256.0 70 217.5 71 227.0 72 195.0 73 146.5 74 116.5 75 103.0 76 87.0 77 70.0 78 56.0 79 43.0 80 51.5 81 46.0 82 26.0 83 20.0 84 16.0 85 9.5 86 7.0 87 7.0 88 7.5 89 7.5 90 4.5 91 5.5 92 9.0 93 6.0 94 14.5 95 29.5 96 33.0 97 44.5 98 104.0 99 1357.0 100 2562.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.035495281575500914 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0036719256802242323 7 0.006119876133707054 8 0.0 9 0.0024479504534828216 10-11 0.0 12-13 0.006119876133707054 14-15 0.0 16-17 0.004283913293594938 18-19 0.0 20-21 0.004895900906965643 22-23 6.119876133707054E-4 24-25 0.0012239752267414108 26-27 0.003059938066853527 28-29 0.0036719256802242323 30-31 0.0012239752267414108 32-33 0.0036719256802242323 34-35 0.0 36-37 0.0 38-39 0.0018359628401121162 40-41 0.007343851360448465 42-43 0.003059938066853527 44-45 0.0036719256802242323 46-47 0.003059938066853527 48-49 0.0 50-51 0.0012239752267414108 52-53 0.0018359628401121162 54-55 0.0036719256802242323 56-57 0.006731863747077759 58-59 0.0036719256802242323 60-61 0.0 62-63 0.0 64-65 0.006731863747077759 66-67 0.0024479504534828216 68-69 0.0036719256802242323 70-71 0.007343851360448465 72-73 0.0 74-75 0.005507888520336348 76 0.013463727494155519 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 81701.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.392516615463702 #Duplication Level Percentage of deduplicated Percentage of total 1 67.96241422333125 21.33511217732953 2 11.221147847785403 7.045201405123561 3 7.310511540860885 6.8848606504204355 4 5.302557704304429 6.658425233473274 5 3.587024329382408 5.630286043010489 6 1.9221771678103554 3.6205187207010927 7 1.1033998752339365 2.4246949241747346 8 0.6784154709918901 1.7037735156240439 9 0.25733000623830316 0.727041284684398 >10 0.5068621334996881 2.708657176778742 >50 0.0779787897691828 1.609527423164955 >100 0.050686213349968805 3.2655659049460843 >500 0.003898939488459139 0.7294892351378808 >1k 0.011696818465377417 9.46744837884481 >5k 0.0 0.0 >10k+ 0.003898939488459139 26.18939792658597 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCCGT 21397 26.18939792658597 No Hit TCTTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCCG 3063 3.749036119508941 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 2616 3.201919193155531 No Hit CTCTTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCC 2056 2.5164930661803404 No Hit ATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCCGTCTT 596 0.7294892351378808 RNA PCR Primer, Index 45 (95% over 22bp) CATATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCCGT 428 0.5238613970453239 No Hit CTTATACACATCTCCGCGCCCACGAGACGCCAATTCATCTCGTATGCCGT 388 0.47490238797566736 No Hit TCTTTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCC 329 0.40268784959792414 No Hit CTAATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCCGT 249 0.3047698314586113 No Hit CTTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGACGT 214 0.26193069852266193 No Hit CTTATACACATCTCCGAGCCCACGAGCCGCCAATTCATCTCGTATGCCGT 170 0.2080757885460398 No Hit CTTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCAGT 153 0.18726820969143584 No Hit CTTATACACATCTCCGAGCCCACGAGACGCCAATTCATATCGTATGCCGT 146 0.178700383104246 No Hit CTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCCGTC 139 0.1701325565170561 No Hit ATTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCCGT 137 0.16768460606357327 No Hit CTTTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCCG 107 0.13096534926133097 No Hit CCTTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCCG 105 0.12851739880784813 No Hit CTTTACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCCGTC 103 0.12606944835436532 No Hit CTTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCCGA 93 0.1138296960869512 No Hit CTTATACACATCTCCGAGCCCACGAGACGCCAATTCCTCTCGTATGCCGT 90 0.11015777040672696 No Hit CTTATACACATCTCCGAGCCCACGAGACGCCAAATCATCTCGTATGCCGT 85 0.1040378942730199 Illumina PCR Primer Index 7 (95% over 21bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.028151430215052446 0.0 2 0.0 0.0 0.0 0.028151430215052446 0.0 3 0.0 0.0 0.0 0.028151430215052446 0.0 4 0.0 0.0 0.0 0.04406310816269079 0.0 5 0.0 0.0 0.0 0.05630286043010489 0.0 6 0.0 0.0 0.0 0.05630286043010489 0.0 7 0.0 0.0 0.0 0.05630286043010489 0.0 8 0.0 0.0 0.0 0.05630286043010489 0.0 9 0.0 0.0 0.0 0.05875081088358772 0.0 10 0.0 0.0 0.0 0.061198761337070536 0.0 11 0.0 0.0 0.0 0.06242273656381195 0.0 12 0.0 0.0 0.0 0.07588646405796746 0.0 13 0.0 0.0 0.0 0.08078236496493311 0.0 14 0.0 0.0 0.0 0.08812621632538158 0.0 15 0.0 0.0 0.0 0.0917981420056058 0.0 16 0.0 0.0 0.0 0.0917981420056058 0.0 17 0.0 0.0 0.0 0.0917981420056058 0.0 18 0.0 0.0 0.0 0.0917981420056058 0.0 19 0.0 0.0 0.0 0.10526186949976132 0.0 20 0.0 0.0 0.0 0.10648584472650273 0.0 21 0.0 0.0 0.0 0.11505367131369261 0.0 22 0.0 0.0 0.0 0.11505367131369261 0.0 23 0.0 0.0 0.0 0.11872559699391684 0.0 24 0.0 0.0 0.0 0.1248454731276239 0.0 25 0.0 0.0 0.0 0.13096534926133094 0.0 26 0.0 0.0 0.0 0.13096534926133094 0.0 27 0.0 0.0 0.0 0.1811483335577288 0.0 28 0.0 0.0 0.0 0.19950796195884996 0.0 29 0.0 0.0 0.0 0.2545868471622134 0.0 30 0.0 0.0 0.0 0.3586247414352334 0.0 31 0.0 0.0 0.0 0.40758375050488976 0.0 32 0.0 0.0 0.0 0.5030538181907198 0.0 33 0.0 0.0 0.0 0.5911800345161013 0.0 34 0.0 0.0 0.0 0.7711043928470888 0.0 35 0.0 0.0 0.0 0.9363410484571792 0.0 36 0.0 0.0 0.0 1.1811360938054614 0.0 37 0.0 0.0 0.0 1.8286189887516677 0.0 38 0.0 0.0 0.0 2.4920135616455124 0.0 39 0.0 0.0 0.0 3.221502796783393 0.0 40 0.0 0.0 0.0 3.878777493543531 0.0 41 0.0 0.0 0.0 4.71964847431488 0.0 42 0.0 0.0 0.0 5.565415355993195 0.0 43 0.0 0.0 0.0 6.413630188124992 0.0 44 0.0 0.0 0.0 7.320595831140378 0.0 45 0.0 0.0 0.0 8.35607887296361 0.0 46 0.0 0.0 0.0 9.23122116008372 0.0 47 0.0 0.0 0.0 10.067196239948103 0.0 48 0.0 0.0 0.0 10.804029326446432 0.0 49 0.0 0.0 0.0 11.589821422014419 0.0 50 0.0 0.0 0.0 12.289935251710505 0.0 51 0.0 0.0 0.0 12.993721007086817 0.0 52 0.0 0.0 0.0 13.69138688632942 0.0 53 0.0 0.0 0.0 14.384156864665059 0.0 54 0.0 0.0 0.0 14.897002484669711 0.0 55 0.0 0.0 0.0 15.415967980808068 0.0 56 0.0 0.0 0.0 16.020611742818325 0.0 57 0.0 0.0 0.0 16.53835326372994 0.0 58 0.0 0.0 0.0 17.060990685548525 0.0 59 0.0 0.0 0.0 17.50039779194869 0.0 60 0.0 0.0 0.0 17.952044650616273 0.0 61 0.0 0.0 0.0 18.368196227708353 0.0 62 0.0 0.0 0.0 18.76843612685279 0.0 63 0.0 0.0 0.0 19.22620286165408 0.0 64 0.0 0.0 0.0 19.571363875595157 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGCCGTC 15 0.002217571 69.99939 45 ATCTCGA 25 2.8175727E-6 69.99939 37 CATGCCA 15 0.002217571 69.99939 26 ACCTGCA 15 0.002217571 69.99939 7 TTGTAAG 15 0.002217571 69.99939 35 TATGAGT 15 0.002217571 69.99939 37 AAGCCGT 15 0.002217571 69.99939 44 CTCGAAT 25 2.8175727E-6 69.99939 39 GACGCAA 20 7.885027E-5 69.99939 26 TGTACTT 15 0.002217571 69.99939 5 GTGACAA 15 0.002217571 69.99939 54 AGGGGGG 2605 0.0 63.953373 70 CGTCTTA 50 1.4551915E-11 62.99945 48 CCGACTT 40 1.0508302E-8 61.249466 47 CGACTTC 40 1.0508302E-8 61.249466 48 CAAATAC 35 2.929355E-7 60.036217 1 CCGTCTA 35 2.941797E-7 59.999477 47 TCTTATG 60 1.8189894E-12 58.332825 50 AATGCCG 30 8.311345E-6 58.332825 43 ACTCGTA 30 8.311345E-6 58.332825 38 >>END_MODULE