FastQCFastQC Report
Wed 25 May 2016
SRR1780874_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780874_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences311632
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG300039.627701904810802No Hit
CTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTG14690.47138933100580166No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCGT150.002222691270.00545520
CGACGTT200.00694516452.50409334
ATTACGA200.006949573852.49566724
CCATGCG200.006949573852.4956673
GGGACTA200.006949573852.4956671
GTTATTC200.006949573852.49566753
ATCTCGG3250.048.4575442
GATCTCG3200.048.12875441
TCGCCGT3350.048.05573752
CTCGGTG3150.047.77383844
GCCGTAT3300.047.7233354
CGGTGGT3450.047.67722746
TGGTCGC3250.047.38070749
GTCGCCG3500.046.99611751
GATCATA305.875162E-446.6628151
GGTATGA305.875162E-446.6628151
AGATCTC3350.045.97373640
TCGGTGG3400.045.2903845
TCTCGGT3400.044.26105543
GTAGATC3600.043.7534138
Produced by FastQC (version 0.11.5)