FastQCFastQC Report
Wed 25 May 2016
SRR1780874_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780874_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences311632
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAATGTCTATCTCGTATGCCGT203946.5442573291574675No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAATGTCTATCTCGTATGCCG30290.9719797710119629No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27270.8750705960876931No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAATGTCTATCTCGTATGCC23140.742542486009139No Hit
ATACACATCTCCGAGCCCACGAGACTAATGTCTATCTCGTATGCCGTCTT7160.2297581763105201RNA PCR Primer, Index 15 (95% over 23bp)
CATATACACATCTCCGAGCCCACGAGACTAATGTCTATCTCGTATGCCGT4390.14087128407865687No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTAATGTCTATCTCGTATGCC3910.12546850130923654No Hit
CTTATACACATCTCCGCGCCCACGAGACTAATGTCTATCTCGTATGCCGT3850.123543153463059No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTGCA150.002224692869.9895719
CGTCTTA207.9254096E-569.9895748
GACTTGC150.002224692869.9895718
AGGGGGG26100.060.0773270
GCAGCGT252.3787317E-456.0456121
ACGAGTA252.3862942E-456.0096325
GAGATCC252.3862942E-456.00963238
CGTATGA900.054.45380841
TCCGGTC200.00694698952.5006078
TGAACGT200.006951452.4921825
GAACGTA200.006951452.4921826
GCCGTCT32200.050.8701546
CGTCTTC32350.050.842548
CCGTCTT31900.050.79995347
TCGTATG33800.050.2305340
TCTTCTG32150.050.17914650
TATGCCG32650.050.0618343
AGCGTCA352.0818221E-550.0085953
CGTATGC33050.049.99346541
CTCGTAT33650.049.9342939