FastQCFastQC Report
Wed 25 May 2016
SRR1780873_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780873_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences395353
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG342328.658591183069307No Hit
CTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTG13440.339949361709662No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG200.00694753952.5021844
CGCGGAT200.00694753952.5021854
TTTCGTC200.006951015452.4955428
TATGCGT200.006951015452.4955470
GATCTTA352.0813377E-550.014731
GCCGTAT3000.047.83532354
TCGCCGT3050.047.0511452
CGCCGTA3350.043.8824253
TGGTCGC3450.043.61948449
CGGTGGT3350.042.83218446
TCGGTGG3450.042.60507645
CTCGGTG3550.042.3961344
GATATTA503.4680415E-642.0123751
GTCGCCG3500.041.99643751
GCGCGTC350.001255293839.9966056
TGCGCGT350.001255293839.9966055
GTAGATC3800.039.62194438
CGTATCA3650.039.31670456
CCGTATC3650.039.31670455
TAGATCT3900.038.5962339
Produced by FastQC (version 0.11.5)