FastQCFastQC Report
Wed 25 May 2016
SRR1780873_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780873_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences395353
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCCGAGATATCTCGTATGCCGT213185.392143223903701No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCCGAGATATCTCGTATGCCG32810.8298912617331853No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25500.6449932086009212No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCCGAGATATCTCGTATGCC19610.4960124243397672No Hit
ATACACATCTCCGAGCCCACGAGACCCCGAGATATCTCGTATGCCGTCTT5680.1436690754844405RNA PCR Primer, Index 31 (95% over 21bp)
CATATACACATCTCCGAGCCCACGAGACCCCGAGATATCTCGTATGCCGT5180.1310221498255989No Hit
CTTATACACATCTCCGAGCCCACGAGACACCGAGATATCTCGTATGCCGT3980.10066952824437908No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAT150.002222849570.0077139
TGACGTC550.063.6433845
AGGGGGG26050.062.6093570
TGCCGTA509.786163E-1056.00617245
GTGTGCG252.3907982E-455.9920143
GCCGTAT452.6951966E-854.45044346
CGTATGA608.367351E-1152.49250841
GCACGTC200.006952604752.49250425
GTAGCGC200.006952604752.49250457
TCGGACG200.006952604752.49250410
TCGCTCG200.006952604752.4925044
TTGCGCA200.006952604752.49250428
ATGCGAT200.006952604752.49250455
ACGTAAT200.006952604752.49250443
GTCGGAC200.006952604752.4925049
GCCGTCA1150.051.74483546
CTTGAAA34800.051.68802657
TCGTATG36250.051.64779740
TCTCGTA36500.051.40292438
CTCGTAT36300.051.3968539