FastQCFastQC Report
Wed 25 May 2016
SRR1780872_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780872_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62924
Sequences flagged as poor quality0
Sequence length76
%GC80

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3370153.558260759010864No Hit
CTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTG15182.412434047422287No Hit
TCTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGT1930.3067192168330049No Hit
CTTATACACATCTGACGCTGCCGCCGATCTACTCTGTGTAGATCTCGGTG1350.2145445299090967No Hit
CTCTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGG1310.20818765494882716No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1290.2050092174686924No Hit
ATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTGGTC1180.18752781132795118No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG780.12395906172525586No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG770.12236984298518848No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG720.11442374928485156No Hit
GGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG630.1001207806242451No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTGGA150.002215113370.057
GTGTCTC150.002215113370.03
GAGTGTC150.002215113370.01
GCTAGGG150.002215113370.032
TCCTAAG150.002215113370.056
GTTTTGG150.002215113370.056
CTAGGGT150.002215113370.033
GTGAGCT150.002215113370.050
AGGGAAC150.002215113370.061
TCACGAT150.002215113370.08
CTTGTGC207.8705874E-570.01
CGCGGCC150.002215113370.016
TAGGGTT150.002215113370.034
GCTCCTA150.002215113370.054
GTGCGCG150.002215113370.04
TACTTTC150.002215113370.038
CTCATGT150.002215113370.045
TCTCATG150.002215113370.044
TTGTGCG207.8705874E-570.02
TGAGCTC150.002215113370.051
Produced by FastQC (version 0.11.5)