SRR1780872_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1780872_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	62924
Sequences flagged as poor quality	0
Sequence length	76
%GC	49

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCGT	22688	36.05619477464878	RNA PCR Primer, Index 14 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCG	3277	5.207869811200814	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	2721	4.324264191723349	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCC	2105	3.345305447841841	RNA PCR Primer, Index 14 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCGTCTT	650	1.0329921810437988	RNA PCR Primer, Index 14 (96% over 26bp)
CTTATACACATCTCCGCGCCCACGAGACACAGTTGCATCTCGTATGCCGT	594	0.9439959316000255	RNA PCR Primer, Index 14 (95% over 23bp)
CATATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCGT	499	0.793020151293624	No Hit
TCTTTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCC	451	0.7167376517703896	RNA PCR Primer, Index 14 (95% over 21bp)
CTAATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCGT	331	0.5260314029623037	No Hit
CTTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGACGT	231	0.3671095289555654	No Hit
CTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCGTC	217	0.3448604665946221	No Hit
ATTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCGT	170	0.2701671858114551	No Hit
CTTATACACATCTCCGAGCCCACGAGACACAGTTGCATATCGTATGCCGT	152	0.24156124849024221	No Hit
CTTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTAAGCCGT	123	0.19547390502828807	No Hit
CCTTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCG	116	0.1843493738478164	No Hit
CTTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCAGT	116	0.1843493738478164	No Hit
CTTATACACATCTCCGAGCCCACGAGACACAGATGCATCTCGTATGCCGT	110	0.17481406140741212	No Hit
CTTTACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCGTC	109	0.17322484266734473	RNA PCR Primer, Index 14 (95% over 24bp)
CTTTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCG	108	0.17163562392727735	No Hit
TATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCGTCT	103	0.16368953022694044	RNA PCR Primer, Index 14 (96% over 25bp)
TCTTATACACATCTCCGCGCCCACGAGACACAGTTGCATCTCGTATGCCG	100	0.1589218740067383	No Hit
CTTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTAGTATGCCGT	89	0.1414404678659971	No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	88	0.1398512491259297	No Hit
TTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCGTC	88	0.1398512491259297	No Hit
CTTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCGA	86	0.13667281164579492	RNA PCR Primer, Index 14 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACACAGTAGCATCTCGTATGCCGT	80	0.12713749920539064	RNA PCR Primer, Index 24 (95% over 23bp)
TCATATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCG	79	0.12554828046532326	RNA PCR Primer, Index 14 (95% over 22bp)
CTTAAACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCGT	76	0.12078062424512111	RNA PCR Primer, Index 14 (95% over 23bp)
CTTATACACATCTCCGAGACCACGAGACACAGTTGCATCTCGTATGCCGT	73	0.11601296802491894	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCC	72	0.11442374928485156	No Hit
CTTATACACATCTCCGAGCCAACGAGACACAGTTGCATCTCGTATGCCGT	70	0.11124531180471679	No Hit
CTTATACACATCTCCGAGCCCACGAGCCACAGTTGCATCTCGTATGCCGT	70	0.11124531180471679	RNA PCR Primer, Index 14 (95% over 23bp)
CTTAATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCG	66	0.10488843684444728	RNA PCR Primer, Index 14 (95% over 22bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTCGAG	15	0.0022082292	70.05489	1
TCAAGGC	15	0.0022152127	69.99921	10
GAGGATC	15	0.0022152127	69.99921	8
TGCCGAC	35	3.6379788E-9	69.99921	45
GTTCCCT	15	0.0022152127	69.99921	46
ATCTACC	15	0.0022152127	69.99921	20
CTCTCCG	15	0.0022152127	69.99921	10
CTTAGGA	15	0.0022152127	69.99921	51
TCACGTA	40	1.3096724E-10	69.99921	38
AAAAGGT	15	0.0022152127	69.99921	67
TGAAGTC	15	0.0022152127	69.99921	45
CTAATGC	15	0.0022152127	69.99921	51
AAGGCAA	15	0.0022152127	69.99921	12
CAAGGCA	15	0.0022152127	69.99921	11
CCCTTAG	15	0.0022152127	69.99921	49
AAGCTTG	15	0.0022152127	69.99921	54
CCGACTT	20	7.8710305E-5	69.99921	47
TTAAATT	15	0.0022152127	69.99921	26
TCCCTTA	15	0.0022152127	69.99921	48
CTCGAGG	15	0.0022152127	69.99921	2

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content