FastQCFastQC Report
Wed 25 May 2016
SRR1780871_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780871_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1063989
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG305322.8695785388758717No Hit
CTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTG17370.1632535674710923No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT3850.042.7306354
TGGTCGC4200.041.66602349
CGCCGTA4250.041.17970353
TCGCCGT4350.040.23304752
GTCGCCG4450.040.1117451
GTGGTCG4750.037.576648
CGTATCA4600.037.2855456
CCGTATC4450.036.96919655
CGGTGGT4950.036.06174546
TCGGTGG5100.035.00110645
CTCGGTG4800.035.00110244
TCTCGGT5300.032.35951243
GGTCGCC5650.030.97151650
GTAGATC6500.026.38544838
GGTGGTC7050.025.31994847
GATCTCG6800.025.2225741
GTATCAT6700.025.076657
CGCTGAT700.001227277424.9972637
ACGTCCG700.001227950124.9949158
GTGTAGA7700.023.63933236
Produced by FastQC (version 0.11.5)