FastQCFastQC Report
Wed 25 May 2016
SRR1780871_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780871_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1063989
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATTTATCTATCTCGTATGCCGT202191.9003016008624147No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTTATCTATCTCGTATGCCG31080.2921082830743551No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30880.29022856439305295No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATTTATCTATCTCGTATGCC18090.17002055472378005No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG28300.054.29623870
TATGCCG32200.051.29749343
CGTCTTC32650.051.23358548
GCCGTCT32400.050.76720446
CCGTCTT32650.050.27129447
ATGCCGT32800.050.25479544
TCGTATG33950.050.20891640
TCTCGTA33550.050.18392638
TGCCGTC33000.050.16232345
CGTATGC33050.050.09349441
CTCGTAT33800.049.91630639
ATCTCGT34200.049.33248537
TATCTCG34300.049.1886636
GTATGCC33850.048.79702442
ATGACGT1250.047.59587544
CTGCTTG35700.045.78895654
AAGGGGG34300.044.59216769
CTTGAAA37350.044.51170757
GTCTTCT37750.044.3119649
CTTCTGC38200.043.43372751
Produced by FastQC (version 0.11.5)