FastQCFastQC Report
Wed 25 May 2016
SRR1780870_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780870_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences253601
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG235559.288212585912516No Hit
CTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTG10080.39747477336445836No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGGCG150.002223769469.9930952
GTCGGGA150.002223769469.9930952
CCGCATG308.36624E-658.3275839
GCCGTAT1400.057.4943354
CCGACGT200.00693769952.51553313
GATTGAC200.006943113652.50517769
TGGTCGC1550.051.9406149
TCGCCGT1550.051.93036752
CTCGGTG1650.050.90407644
GTCGCCG1600.050.3174651
GATCTCG1600.050.3075441
CGGTGGT1800.048.61590246
ATCTCGG1700.047.34827442
AGATCTC1700.047.34827440
TCGGTGG1850.047.3019645
CGTATCA1800.046.66206756
AAGGCGT305.8735465E-446.66206723
GTAGATC1800.044.7266338
CGCCGTA1800.044.71781553
TCTCGGT2150.043.94915843
Produced by FastQC (version 0.11.5)