FastQCFastQC Report
Wed 25 May 2016
SRR1780870_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780870_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences253601
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGTCCGCGATCTCGTATGCCGT128575.0697749614552No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGTCCGCGATCTCGTATGCCG23240.9164001719236123No Hit
ATTATACACATCTCCGAGCCCACGAGACTGTCCGCGATCTCGTATGCCGT18040.7113536618546457No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18010.7101707012196324No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGTCCGCGATCTCGTATGCC9230.3639575553724157No Hit
CTTAAACACATCTCCGAGCCCACGAGACTGTCCGCGATCTCGTATGCCGT4610.1817816175803723No Hit
ATACACATCTCCGAGCCCACGAGACTGTCCGCGATCTCGTATGCCGTCTT4020.15851672509177803TruSeq Adapter, Index 1 (95% over 21bp)
CTTATACACATCTCCGCGCCCACGAGACTGTCCGCGATCTCGTATGCCGT2760.10883237842122073No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTGTCCGCGATCTCGTATGCC2670.10528349651618094No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG20200.059.4238370
CGAACTC553.6379788E-1157.277435
AATCGGC252.3888359E-455.9935323
AACTCGT452.6906491E-854.4488937
CGTATGA452.6948328E-854.43815641
CCGCGAA653.6379788E-1253.83993532
TCCATTC200.006943576552.5042919
TTGTACG200.006943576552.504299
GCCGTCA800.052.5042946
TATGCCG24300.051.269843
TCTCGTA23900.051.2594238
ATCTCGT23500.051.2382337
CTTGAAA24000.051.18158757
GCCGTCT24150.051.163646
TCGTATG25000.051.09409740
CGTATGC24800.051.0828141
CTCGTAT24400.051.06974439
TGACGTC552.2737368E-950.91324645
CGTCTTC24200.050.76861248
CTGCTTG23100.050.75171354