FastQCFastQC Report
Wed 25 May 2016
SRR1780869_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780869_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences354197
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG236446.67538121440893No Hit
CTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTG9250.26115410350737017No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTG200.006946422652.5031783
GATCTAA350.001254218940.0024221
CGGTGGT2300.033.4755646
TCGGTGG2200.033.40639545
TCGCCGT1800.033.05755652
GCCGTAT1700.032.94317254
CGCCGTA1750.032.00193853
TAGATCT2100.031.66858539
GACGTAT450.004303231.11299554
TCGATTT450.004309152331.1042128
TGGTCGC2150.030.92773649
GTAGATC2200.030.22910338
AGATCTC2250.029.55734640
GCCGTCC604.9838825E-429.16019827
TCTCGGT2450.028.56912243
CTTATGT500.00720161128.00169456
GGTGGTC2800.027.49778247
CTCGGTG2550.027.44876544
CCGTATC2100.026.66828255
CGTATCA2450.025.71584156
Produced by FastQC (version 0.11.5)